Evaluation of Reactivity Using Direct Assay Methods for High Density Lipoprotein Cholesterol

概要

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We evaluated the lipoprotein specificity of two direct methods based on different principles for quantifying high-density lipoprotein cholesterol (HDL-C). Reagent a, utilizing polyethlene glycol-modified enzymes and sulfated α-cyclodextrin, showed about 6% crossreactivity for very low-density lipoprotein (VLDL), while reagent b which consisted of polyanion and dispersive surfactant showed about 5% crossreactivity for low-density lipoprotein (LDL). There was difference in the reactivity for HDL3 among the two direct methods and the precipitation method, but both direct methods exhibited a higher cholesterol value for HDL2 than the precipitation method. To investigate the reactivity to HDL2 in detail, the HDL2 fraction was separated into HDL with apo E and HDL without apo E by heparin-sepharose affinity chromatography. The precipitation method measured only HDL without apo E, but HDL-C measured by the two direct methods included both of HDL with and without apo E. HDL-C values by the direct method were in agreement with the values of total cholesterol in HDL fractions isolated by ultracentrifugation.
Japan Society of Clinical Chemistryの論文