Evaluation of Reaction for Slow a-Lipoproteins Cholesterol in Sera Using the Direct, Precipitation and Gel-Filtration Chromatography Method

概要

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The direct method and the precipitation-based method of high density lipoprotein-cholesterol (HDL-C) assay demonstrated different HDL-C levels for two serum samples, one with a high level of apolipoprotein E (apoE) and one containing slow-alpha lipoprotein. We compared HDL-cholesterol reaction for three serum samples with a high apoE level, slow-alpha lipoprotein, and normal sera using the direct, precipitation and gel filtration chromatography methods. There were three patterns detected in the samples: (1) Samples showing correlation on all three methods,(2) Samples showing correlation between the direct and the precipitation method, but no correlation between the direct method and gel filtration chromatography, and (3) Samples showing no correlation among the three methods. These results suggests that abnormal HDL with large particle size includes three or more kinds of apoE-rich HDL or slow-alfa lipoproteins with different origins or hysiological and chemical characteristics.
Japan Society of Clinical Chemistryの論文