Gas Chromatographic Analysis for Anticonvulsant Drugs in Biological Fluids
スポンサーリンク
概要
- 論文の詳細を見る
A gas-liquid chromatographic method for the simultaneous determination of phenobarbital (PB), carbamazepin (Carb), primidone (Prim) and diphenylhydantoin (DPH) in human biological fluids following therapeutic doses has been described.<BR>After extraction with chloroform, the anticonvulsant drugs were methylated with phenyl-trimethylammonium hydroxide in dimethylformamide at 85° for 10 min. Liner temperature programming of a 1% OV-17 column was used to achieve separation and quatitation. The procedure described in the present paper is relatively simple, highly specific and sufficiently sensitive for use in routine clinical assays.<BR>The anticonvulsant drug levels were measured not only in plasma but in saliva. Furthermore, the unbinding drugs in plasma were measured by means of ultrafiltration techniques. Ultrafiltration with using plasma aliquots (1 ml) was performed by an Amincon Centriflo membran cone (CF50) at 20°, 1,000 g for 25 min. It is applicable to screening large samples of patients.<BR>Although it was found a patient having plasma level of 25 μg/ml, which has been considered to be the upper limit of the therapeutic range for DPH, the unbinding fraction in plasma was only 0.7 μg/ml. Thus, it should be noted that a knowledge of unbinding drugs would be more desirable for a therapeutic level than the total drugs level in plasma.
- Japan Society of Clinical Chemistryの論文
著者
関連論文
- 気管支喘息における血中Theophylline濃度測定に関する臨床的検討
- 血清酵素の変動に依り診断し得た急性心筋硬塞症の2症例 : 第67回日本循環器学会関東甲信越地方会
- 大動脈弁狭窄症を合併せる家族性高コレステロール血症性黄色腫症の1症例 : 第57回日本循環器学会関東甲信越地方会
- ロ-220 腫瘍マーカーと日常検査項目分析値の多変量解析による肺癌スクリーニングの試み
- 臨床検査器械器具の近代化
- 癌性胸膜炎における胸水LDHアイソザイム : 第14回日本肺癌学会総会演題抄録
- 癌性肋膜炎と非癌性肋膜炎の鑑別診断 : (III)肺癌の早期診断
- 557 血液成分の多変量解析による大腸癌スクリーニングの試み(第26回日本消化器外科学会総会)
- 153 血液成分の多変量解析法による大腸癌スクリーニングの試み(第22回日本消化器外科学会総会)
- 388 気管支喘息における, 血中Theophylline濃度測定の意義に関する臨床的検討