Measurement of uric acid by the FUJI multilayer film system

概要

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We evaluated the analytical performance of the FUJI DRI- CHEM multilayer film system for the determination of uric acid in serum and urine. The hydrogen peroxide produced from uric acid, is oxidized by uricase. It is oxidatively reacted with leuco dye in the present of peroxidase to yield a chromogen with an absorption maximum at 640nm. Results are obtained 6 min. after receipt the sample. The intra-assay coefficient of variation (CV) was 1.3-2.4% (n= 20) for uric acid concentrations of 2-12mg/dl, inter-assay CV was 1.3-2.9% (n= 20) at 2-11mg/dl. The response of the method varied linearity with uric acid concentrations up to 9mg/dl. The results agreed well with those by the uricase- catalase method (p<0.001). We concluded that the method is convenient, rapid and simple and thus is appropriate for use in emergency rooms and small laboratories.
Japanese Society of Gout and Nucleic Acid Metabolismの論文