Heat-8table Alkaline Phosphatase Isoenzyme (Nagao Isoenzyme) Detected in Adenocarcinoma of Pancreas Tail
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Heat-stable isoenzyme of aikaline phosphatase was detected in the serum of a patient with primary adenocarcinoma of the pancreas tail with the intraperitoneal metastasis. On autopsy, tissue specimens including metastasis in the greater omentum were subjected to the study of heat-stability, sensitivity to L-leucine or EBTA,and to the anaiysis of enzyme kinetics.<BR>It was revealed that the heat-stable isoenzyme was detected only in tumor tissues,peritoneal fluid or in serum. Furthermore, the heat-stable isoenzyme showed increased sensitivity to L-leucine and enzyme kinetics similar to those obtained in Nagao isoenzyme (Nakayama et al: Clin-Chim-Acta, 30, 546, 1970). This is the first description of this type of alkaline phosphatase in tumor tissue specimens.
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