Polypeptide synthesis using the S-alkyl thioester of a partially protected peptide segment. Synthesis of the DNA-binding domain of c-Myb protein(142-193)-NH2.
スポンサーリンク
概要
- 論文の詳細を見る
A method for polypeptide synthesis using <I>S</I>-alkyl thioester of a partially protected peptide segment has been developed. The DNA-binding domain of <I>c</I>-Myb protein (142–193) was synthesized by this method to estimate its usefulness. The partially protected peptide segments, Boc–[Lys(Boc)<SUP>143,144,160</SUP>]–<I>c</I>-Myb protein(142–163)–SCH<SUB>2</SUB>CH<SUB>2</SUB>CONH<SUB>2</SUB> and [Lys(Boc)<SUP>171,182,192</SUP>]–<I>c</I>-Myb protein(164–193)–NH<SUB>2</SUB>, were highly purified from peptides obtained by a solid-phase method. The thioester in the carboxyl component of the peptide segment was converted to the corresponding <I>p</I>-nitrophenyl ester in the presence of silver ions and <I>p</I>-nitrophenol and then the two segments were condensed. The coupling reaction proceeded almost completely yielding a highly-purified product after removal of Boc groups.
- 公益社団法人 日本化学会の論文
著者
-
Aimoto Saburo
Institude For Protein Research Osaka University
-
Hojo Hironobu
Institute For Protein Research Osaka University
関連論文
- Development of a Method for the Preparation of Peptide Thioesters by an Fmoc Solid Phase Method
- Synthesis of Cysteine-Containing Polypeptide Using a Peptide Thioester Containing a Cys(Acm) Residue
- Polypeptide Synthesis Using Cys(Acm)-Containing Peptide Thioester as Building Block
- Synthesis of a Partial Sequence of Site-Specifically Methylated Histone H3
- Peptide Purification Using α-Ketoacyl Group Chemistry
- Preparation of a Peptide Thioester by the Fmoc-Solid Phase Method via an On-Resin N-S Acyl Shift
- Chemical Ligation Using a Photoremovable Auxiliary
- Interaction of Synthetic J Protein of Bacteriophage ΦK with Its Circular Single-Stranded DNA
- Synthesis of Transmembrane Protein, F_1F_0 ATP Synthase Subunit c, by Thioester Method
- Chemical Synthesis of H^+-ATP Synthase Subunit c
- Peptide Synthesis Mediated by Thiolsubtilisin Using Peptide Thioester as Building Block
- The N-Terminal Helical Segments Modulate Structure and Lipid Interaction of Human Apolipoprotein A-I
- Chemical Synthesis of the Extracellular Juxtamembrane-Transmembrane Region of the Thrombopoietin Receptor
- Peptide Thioester Synthesis Based on an N-S Acyl Shift Reaction
- 2P332 Interaction of Arrestin with Phosphorylated Rhodopsin Cterminus Peptide(42. Sensory signal transduction,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Studies on Structure-Activity Relationship of HTLV-1 Protease Inhibitors
- Solid-Phase Syntheses of Olefin-Containing Protease Inhibitors Using Horner-Emmons Reaction
- Synthesis of Opioid Receptor Like 1 (251-370) by Combining the Native Chemical Ligation and the Thioester Method
- Synthesis of Olefin-Containing Cyclic Peptide Library Using the Solid-Phase Horner-Emmons Reaction
- Solid-Phase Peptide Synthesis Using N^α-Fmoc(2-F)-Amino Acid and Its Application to the Synthesis of Phosphorylated Peptide Thioesters
- Total Synthesis of Human T-cell Leukemia Virus Type-1 Protease via the Thioester Method
- Synthesis of a Molt-Inhibiting Hormone of the American Crayfish, Procambarus clarkii and Determination of Its Disulfide Linkage
- Synthesis of Phosphorylated Polypeptide Using an Expressed Peptide as a Building Block
- Changes in the Amounts of the Molt-Inhibiting Hormone in Sinus Glands during the Molt Cycle of the American Crayfish, Procambarus clarkii : Endocrinology
- Synthesis of a Molt-Inhibiting Hormone of the American Crayfish, Procambarus clarkii, and Determination of the Location of Its Disulfide Linkages^1
- Use of an Expressed Peptide Segment as a Building Block for the Preparation of Phosphorylated Polypeptide via the Thioester Method
- Synthesis of a Phosphorylated Polypeptide by a Thioester Method
- SYNTHESIS OF A MOLT - INHIBITING HORMONE IN THE AMERICAN CRAYFISH PROCAMBARUS CLARKII BY NATIVE CHEMICAL LIGATION
- Synthesis of Cysteine-Containing Polypeptide Using Peptide Thioester : Preparation of a Molt-Inhibiting Hormone from the American Crayfish, Procambarus clarkii
- Peptide Ligation Using a Building Block Having a Cysteinyl Prolyl Ester (CPE) Autoactivating Unit at the Carboxy Terminus
- Sequential Peptide Ligation by the Combination of the Thioester Method and Extended Chemical Ligation
- N-S Acyl Shift Reaction on Peptide Backbone and Its Application to Preparation of Peptide Thioesters
- Peptide Ligation That Involves an Intramolecular N to S Acyl Shift Reaction
- Generation of an S-Peptide via an N-S Acyl Shift Reaction in a TFA Solution
- Preparation of Peptide Thioesters Based on an N-S Acyl Shift Reaction
- Xenopus Eggs Express an Identical DNA Methyltransferase, Dnmtl, to Somatic Cells
- Molecular Mechanism of Liposome Membrane Fusion Induced by Amphipathic Helical Peptides
- Isolation of Bioactive Peptides from Wamide-Peptide Libraries Synthesized with a Multipeptide Synthesizer
- Isolation of Neurotensin-related Bioactive Pentapeptides from Peptide Libraries Synthesized with a Multipeptide Synthesizer
- Preparation of S-Protected Cysteine-Containing Peptide Thioester and Its Use for the Synthesis of the Barnase-Like Domain in DNA-Directed RNA Polymerase II of Saccharomyces cerevisiae
- Synthesis of β-Peptides utilizing C-Glycosyl β-Amino Acid
- Peptide Ligation Using a Photoremovable Auxiliary
- Characterization of the Ligand-Binding Specificities of Integrin α3β1 and α6β1 Using a Panel of Purified Laminin Isoforms Containing Distinct α Chains
- Structure of the Transmembrane : Juxtamembrane Region of Amyloid Precursor Protein
- Structure of the Membrane-Reconstituted Transmembrane-Juxtamembrane Peptide, Neu(647-693), and Its Interaction with Ca^/Calmodulin
- Structural Analysis of the Transmembrane-Juxtamembrane Region of FGFR3
- Structure of the Transmembrane - Juxtamembrane Sequence of EGFR and Its Interaction with Ca^/Calmodulin
- Characterization of Lipid-Binding Domains in Human Serum Amyloid A Using Its Fragment Peptides
- Peptide Purification Using The Selective Reaction Between Aminooxy Group and Isothiocyanate Group, Followed by Edman Degradation
- Methylthio Groups for Thiol Protection that Realize the Sequential Ligation by Native Chemical Ligation Followed by Thioester Method
- 2-Nitrobenzylcarbamate-type Photocleavable Linker Having Fmoc-aminoalkyloxy Group : Preparation and Application to Peptide Purification
- Synthesis of Voltage-Dependent Proton Channel (VSOP)(121-222)
- Synthesis of Core-class 2 O-Linked Glycopeptides : a Benzyl-protected Tetrasaccharyl Serine and its Derivative Carrying a Hydrophobic Cholestanyl Group(Organic Chemistry)
- Structure-Activity Relation of LWamide Peptides Synthesized with a Multipeptide Synthesizer
- An Antagonist of Molluscan Neuropeptide APGW amide Found in a Peptide Library
- New Phosphoryl Protecting Groups for Synthesis of Phosphopeptides by Boc Method
- Stereoselective Synthesis and Evaluation of HTLV-1 Protease Inhibitors
- Preparation of Partially Protected Peptide Thioesters Containing a Cysteine Residue and Their Segment Condensation
- Synthesis of an Unnatural N-Glycan-linked Dolichyl Pyrophosphate Precursor(Organic Chemistry)
- New Redox Linker for the Preparation of Subunit c of Human F_1F_O-ATP Synthase
- Condensation of Cys(Acm)-Containing Peptide Segments with Silver Chloride as an Activator of Peptide Thioesters
- Peptide Thioester Formation by an N to S Acyl Shift Reaction at the Cysteinyl Prolyl Cysteine Position
- Synthesis of Peptide Thioester by the Cysteinyl Prolyl Ester (CPE) Method
- Chemical Synthesis of the Functional Domain of Voltage-Gated Proton Channel
- Preparation of Peptide Thioester by Fmoc Solid-Phase Method
- Peptide Ligation via Thioester Formation at the Cysteine Residue
- Solid State NMR study on the Dimer Structure of the Transmembrane Region of Amyloid Precursor Protein
- Study for Serotonin Release-Modulating Action by Molluscan Neuropeptide APGWamide Using an Antagonist
- Development of Potent Peptidic Analogues of APGWGNamide ; The Antagonist of Molluscan Neuropeptide APGWamide
- Solution Structure of a Peptide Designed of Plastocyanin Metal Center in Trifluoroethanol and Incorporation of Cobalt(II) into the Peptide
- Synthesis of Cyclic RGD Derivatives on Solid Support
- Structural Difference between the Wild Type and the Constitutively Active Mutant Transmembrane-Juxtamembrane Regions of Receptor Tyrosine Kinase Revealed by Solid State NMR and Fluorescence Spectroscopy
- Formation of Peptide Thioesters by the Use of Modified Cys-Pro Ester (CPE) Autoactivating Unit
- Physicochemical Mechanism for the Lipid Membrane Binding of Polyarginine : The Favorable Enthalpy Change with Structural Transition from Random Coil to α-Helix
- Development of a Chemical Protein-Synthesis Method
- Computation of amino acid sequences of polypeptides from masses of their constituent peptide fragments and amino acid residues released in Edman-degradation.
- A new method for carboxyl-terminal sequence analysis of a peptide using carboxypeptidases and field-desorption mass spectrometry.
- A Physico-Chemical Study of the DNA-Binding Domain of GATA1.
- Chemical synthesis of a heat-stable enterotoxin produced by enterotoxigenic Escherichia coli strain 18D.
- Structure-activity relationship of a heat-stable enterotoxin produced by Yersinia enterocolitica.
- Development of a facile method for polypeptide synthesis. Synthesis of bovine pancreatic trypsin inhibitor (BPTI).
- Polypeptide synthesis using the S-alkyl thioester of a partially protected peptide segment. Synthesis of the DNA-binding domain of c-Myb protein(142-193)-NH2.
- Regeneration to the native form of hen egg-white lysozyme from its protected derivatives.
- Chemical synthesis of fully active and heat-stable fragments of heat-stable enterotoxin of enterotoxigenic Escherichia coli strain 18D.
- Influence of Anhydrous Hydrogen Fluoride on Hen Egg-White Lysozyme. I. Effects on Native Hen Egg-White Lysozyme
- Synthesis of a heat-stable enterotoxin (STh) produced by a human strain SK-1 of enterotoxigenic Escherichia coli.
- Heat-stable enterotoxin (STh) of human enterotoxigenic Escherichia coli (strain SK-1). Structure-activity relationship.
- Sequential Peptide Ligation by Combining the Cys-Pro Ester (CPE) and Thioester Methods and Its Application to the Synthesis of Histone H3 Containing a Trimethyl Lysine Residue
- Sequential Peptide Ligation by the Combination of Cys-Pro Ester and Thioester Methods : An Application to a Synthesis of Histone H3