Sequential Peptide Ligation by the Combination of Cys-Pro Ester and Thioester Methods : An Application to a Synthesis of Histone H3

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概要

• 論文の詳細を見る
• 2013-03-01

著者

• Kawakami Toru

  Institute For Protein Research Osaka University

• Akai Yuichi

  Institute For Protein Research Osaka University

• Kita Chieko

  Institute For Protein Research Osaka University

• Takemura Lisa

  Institute For Protein Research Osaka University

• Aimoto Saburo

  Institude For Protein Research Osaka University

• Fujimoto Hisao

  Institute For Protein Research Osaka University

• AOKI Yuko

  Institute for Protein Research, Osaka University

• Waseda Masazumi

  Institute For Protein Research Osaka University

関連論文

• Development of a Method for the Preparation of Peptide Thioesters by an Fmoc Solid Phase Method
• Conglomerate of the Karaumedate Formation in the Kitakami Massif, Northeast Japan
• Synthesis of Cysteine-Containing Polypeptide Using a Peptide Thioester Containing a Cys(Acm) Residue
• Polypeptide Synthesis Using Cys(Acm)-Containing Peptide Thioester as Building Block
• Synthesis of a Partial Sequence of Site-Specifically Methylated Histone H3
• Peptide Purification Using α-Ketoacyl Group Chemistry
• Preparation of a Peptide Thioester by the Fmoc-Solid Phase Method via an On-Resin N-S Acyl Shift
• Chemical Ligation Using a Photoremovable Auxiliary
• Interaction of Synthetic J Protein of Bacteriophage ΦK with Its Circular Single-Stranded DNA
• Synthesis of Transmembrane Protein, F_1F_0 ATP Synthase Subunit c, by Thioester Method
• Chemical Synthesis of H^+-ATP Synthase Subunit c
• Peptide Synthesis Mediated by Thiolsubtilisin Using Peptide Thioester as Building Block
• The N-Terminal Helical Segments Modulate Structure and Lipid Interaction of Human Apolipoprotein A-I
• Chemical Synthesis of the Extracellular Juxtamembrane-Transmembrane Region of the Thrombopoietin Receptor
• Peptide Thioester Synthesis Based on an N-S Acyl Shift Reaction
• 2P332 Interaction of Arrestin with Phosphorylated Rhodopsin Cterminus Peptide(42. Sensory signal transduction,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Studies on Structure-Activity Relationship of HTLV-1 Protease Inhibitors
• Solid-Phase Syntheses of Olefin-Containing Protease Inhibitors Using Horner-Emmons Reaction
• Synthesis of Opioid Receptor Like 1 (251-370) by Combining the Native Chemical Ligation and the Thioester Method
• Synthesis of Olefin-Containing Cyclic Peptide Library Using the Solid-Phase Horner-Emmons Reaction
• Solid-Phase Peptide Synthesis Using N^α-Fmoc(2-F)-Amino Acid and Its Application to the Synthesis of Phosphorylated Peptide Thioesters
• Total Synthesis of Human T-cell Leukemia Virus Type-1 Protease via the Thioester Method
• Synthesis of a Molt-Inhibiting Hormone of the American Crayfish, Procambarus clarkii and Determination of Its Disulfide Linkage
• Synthesis of Phosphorylated Polypeptide Using an Expressed Peptide as a Building Block
• Changes in the Amounts of the Molt-Inhibiting Hormone in Sinus Glands during the Molt Cycle of the American Crayfish, Procambarus clarkii : Endocrinology
• Synthesis of a Molt-Inhibiting Hormone of the American Crayfish, Procambarus clarkii, and Determination of the Location of Its Disulfide Linkages^1
• Use of an Expressed Peptide Segment as a Building Block for the Preparation of Phosphorylated Polypeptide via the Thioester Method
• Synthesis of a Phosphorylated Polypeptide by a Thioester Method
• SYNTHESIS OF A MOLT - INHIBITING HORMONE IN THE AMERICAN CRAYFISH PROCAMBARUS CLARKII BY NATIVE CHEMICAL LIGATION
• Synthesis of Cysteine-Containing Polypeptide Using Peptide Thioester : Preparation of a Molt-Inhibiting Hormone from the American Crayfish, Procambarus clarkii
• Peptide Ligation Using a Building Block Having a Cysteinyl Prolyl Ester (CPE) Autoactivating Unit at the Carboxy Terminus
• Sequential Peptide Ligation by the Combination of the Thioester Method and Extended Chemical Ligation
• N-S Acyl Shift Reaction on Peptide Backbone and Its Application to Preparation of Peptide Thioesters
• Peptide Ligation That Involves an Intramolecular N to S Acyl Shift Reaction
• Generation of an S-Peptide via an N-S Acyl Shift Reaction in a TFA Solution
• Preparation of Peptide Thioesters Based on an N-S Acyl Shift Reaction
• Xenopus Eggs Express an Identical DNA Methyltransferase, Dnmtl, to Somatic Cells
• Molecular Mechanism of Liposome Membrane Fusion Induced by Amphipathic Helical Peptides
• Isolation of Bioactive Peptides from Wamide-Peptide Libraries Synthesized with a Multipeptide Synthesizer
• Isolation of Neurotensin-related Bioactive Pentapeptides from Peptide Libraries Synthesized with a Multipeptide Synthesizer
• Preparation of S-Protected Cysteine-Containing Peptide Thioester and Its Use for the Synthesis of the Barnase-Like Domain in DNA-Directed RNA Polymerase II of Saccharomyces cerevisiae
• Synthesis of β-Peptides utilizing C-Glycosyl β-Amino Acid
• Polypeptide Synthesis Based on Ligation Chemistry : Development of Auxiliaries for Peptide Ligation
• Peptide Ligation Using a Photoremovable Auxiliary
• Characterization of the Ligand-Binding Specificities of Integrin α3β1 and α6β1 Using a Panel of Purified Laminin Isoforms Containing Distinct α Chains
• Structure of the Transmembrane : Juxtamembrane Region of Amyloid Precursor Protein
• Structure of the Membrane-Reconstituted Transmembrane-Juxtamembrane Peptide, Neu(647-693), and Its Interaction with Ca^/Calmodulin
• Structural Analysis of the Transmembrane-Juxtamembrane Region of FGFR3
• Structure of the Transmembrane - Juxtamembrane Sequence of EGFR and Its Interaction with Ca^/Calmodulin
• Characterization of Lipid-Binding Domains in Human Serum Amyloid A Using Its Fragment Peptides
• Peptide Purification Using The Selective Reaction Between Aminooxy Group and Isothiocyanate Group, Followed by Edman Degradation
• Methylthio Groups for Thiol Protection that Realize the Sequential Ligation by Native Chemical Ligation Followed by Thioester Method
• 2-Nitrobenzylcarbamate-type Photocleavable Linker Having Fmoc-aminoalkyloxy Group : Preparation and Application to Peptide Purification
• Synthesis of Voltage-Dependent Proton Channel (VSOP)(121-222)
• Structure-Activity Relation of LWamide Peptides Synthesized with a Multipeptide Synthesizer
• An Antagonist of Molluscan Neuropeptide APGW amide Found in a Peptide Library
• New Phosphoryl Protecting Groups for Synthesis of Phosphopeptides by Boc Method
• Stereoselective Synthesis and Evaluation of HTLV-1 Protease Inhibitors
• Preparation of Partially Protected Peptide Thioesters Containing a Cysteine Residue and Their Segment Condensation
• New Redox Linker for the Preparation of Subunit c of Human F_1F_O-ATP Synthase
• Condensation of Cys(Acm)-Containing Peptide Segments with Silver Chloride as an Activator of Peptide Thioesters
• Peptide Thioester Formation by an N to S Acyl Shift Reaction at the Cysteinyl Prolyl Cysteine Position
• Synthesis of Peptide Thioester by the Cysteinyl Prolyl Ester (CPE) Method
• Chemical Synthesis of the Functional Domain of Voltage-Gated Proton Channel
• Preparation of Peptide Thioester by Fmoc Solid-Phase Method
• Peptide Ligation via Thioester Formation at the Cysteine Residue
• Solid State NMR study on the Dimer Structure of the Transmembrane Region of Amyloid Precursor Protein
• Study for Serotonin Release-Modulating Action by Molluscan Neuropeptide APGWamide Using an Antagonist
• Development of Potent Peptidic Analogues of APGWGNamide ; The Antagonist of Molluscan Neuropeptide APGWamide
• Solution Structure of a Peptide Designed of Plastocyanin Metal Center in Trifluoroethanol and Incorporation of Cobalt(II) into the Peptide
• Synthesis of Cyclic RGD Derivatives on Solid Support
• Structural Difference between the Wild Type and the Constitutively Active Mutant Transmembrane-Juxtamembrane Regions of Receptor Tyrosine Kinase Revealed by Solid State NMR and Fluorescence Spectroscopy
• Formation of Peptide Thioesters by the Use of Modified Cys-Pro Ester (CPE) Autoactivating Unit
• Physicochemical Mechanism for the Lipid Membrane Binding of Polyarginine : The Favorable Enthalpy Change with Structural Transition from Random Coil to α-Helix
• Development of a Chemical Protein-Synthesis Method
• Computation of amino acid sequences of polypeptides from masses of their constituent peptide fragments and amino acid residues released in Edman-degradation.
• A new method for carboxyl-terminal sequence analysis of a peptide using carboxypeptidases and field-desorption mass spectrometry.
• A Physico-Chemical Study of the DNA-Binding Domain of GATA1.
• Chemical synthesis of a heat-stable enterotoxin produced by enterotoxigenic Escherichia coli strain 18D.
• Structure-activity relationship of a heat-stable enterotoxin produced by Yersinia enterocolitica.
• Development of a facile method for polypeptide synthesis. Synthesis of bovine pancreatic trypsin inhibitor (BPTI).
• Polypeptide synthesis using the S-alkyl thioester of a partially protected peptide segment. Synthesis of the DNA-binding domain of c-Myb protein(142-193)-NH2.
• Regeneration to the native form of hen egg-white lysozyme from its protected derivatives.
• Chemical synthesis of fully active and heat-stable fragments of heat-stable enterotoxin of enterotoxigenic Escherichia coli strain 18D.
• Influence of Anhydrous Hydrogen Fluoride on Hen Egg-White Lysozyme. I. Effects on Native Hen Egg-White Lysozyme
• Synthesis of a heat-stable enterotoxin (STh) produced by a human strain SK-1 of enterotoxigenic Escherichia coli.
• Heat-stable enterotoxin (STh) of human enterotoxigenic Escherichia coli (strain SK-1). Structure-activity relationship.
• Sequential Peptide Ligation by Combining the Cys-Pro Ester (CPE) and Thioester Methods and Its Application to the Synthesis of Histone H3 Containing a Trimethyl Lysine Residue
• Sequential Peptide Ligation by the Combination of Cys-Pro Ester and Thioester Methods : An Application to a Synthesis of Histone H3

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