抽出溶媒にベンゼン及びトルエンを用いない魚介類中のアルキル水銀分析法の検討

概要

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The concentrations of methylmercury (MeHg) and ethylmercury (EtHg) were determined in fish and shellfish. Both MeHg and EtHg were isolated by acid leaching (KBr-H2SO4-CuSO4); MeHg and EtHg bromides were extracted into an organic solvent [cyclohexane/ethyl acetate (1 : 1)], then reextracted into 1% cysteine-sodium acetate. MeHg and EtHg bromides were derivatized with sodium tetraphenylborate (NaBPh4), and extracted with hexane. The organic phase containing methylphenylmercury and ethylphenylmercury was injected into GC/MS (SIM). As a result, even if we did not use benzene or toluene for the extracting solvent, it became possible to extract MeHg and EtHg well. The performance evaluation results, trueness (%), repeatability (RSD%) and reproducibility within the laboratory (RSD%), were MeHg 106.4, 8.9, 12.1 and EtHg 87.4, 8.3, 7.6, respectively. It was shown that the method satisfies the performance criteria set by Ministry of Health, Labor and Welfare.