高速液体クロマトグラフィーによる水中のオキシン銅の定量

概要

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A method for the quantitative determination of oxine-copper [bis (quinolin-8-olato) copper], a fungicide used widely in golf links, in water was developed. Oxine-copper was extracted from the sample water with dichloromethane. The dichloromethane solution was evaporated to dryness. The residue was dissolved in methanol and the solution analyzed by high performance liquid chromatography. The analytical conditions were as follows : column, Inertsil ODS-2 equipped with ceramic filter (GL Sciences, 5 μm, 250 mm×4.6 mm i.d.) ; mobile phase, acetonitrile-50 mM phosphate buffer [pH 3.5,containing 0.1% ethylene-diaminetetraacetic acid disodium salt] (EDTA-2Na) (40 : 60,v/v) ; flow rate, 0.8 ml/min ; detection wavelength, 240 nm. Under these conditions oxine-copper was converted to 8-hydroxyquinoline by EDTA added to the mobile phase, and the resulting 8-hydroxyquinoline determined quantitatively. The calibration curve for oxine-copper was linear in the range of 1-100 ng. The minimum detectable amount of oxine-copper was 0.1 ng. This method is widely applicable to the determination of trace amounts of oxine-copper in water.
社団法人日本薬学会の論文
1991-06-30