ニジマスにおけるアスコルビン酸-2-リン酸の腸管吸収および加水分解活性〔英文〕

概要

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Intestinal absorption of L-ascorbyl-2-phosphate (AsP) in vitro and the activity of enzymatic hydrolysis of AsP to L-ascorbic acid (AsA) were investigated in rainbow trout Oncorhynchus mykiss. AsP magnesium salt or AsA sodium salt as control (each 26 μmol in 0.5 ml of Ringer solution) was injected into intestines removed from rainbow trout. The intestine was ligatured both ends and suspended in Ringer solution with aeration. AsP and AsA transported from the mucosal side to the serosal side were measured by high-performance liquid chromatography. AsP passed through the intestine and part of the AsP was detected as AsA in the serosal fluid. The total quantity of ascorbate (AsA+AsP) transported was the same as that of the control injected sodium AsA. 2, 4-Dinitrophenol (1 mM) inhibited the transportation of AsP and AsA by more than 50%, suggesting the acitive transport mechanism in AsP and AsA absorption from the intestine of rainbow trout. Enzymatic hydrolysis of AsP to AsA investigated using the homogenate of several tissues was highly active in the pyloric caeca and the intestine at alkaline pHs and in the liver at acidic pHs. These findings suggest the ready conversion of AsP to AsA in the rainbow trout body.
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