実験的結核感染組織における脂質変化 : 1.マウス肺組織ならびにマクロファージにおける観察

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With the progress of tuberculous infection in mice, the lung weight increased due to the formation of numerous granulomatous lesions. During the 5 week course of infection, the total lipid amount extractable by chloroform-methanol (2: 1, v/v) from the organ increased continuously up to 3 fold level. Per cent distribution of each lipid in the extract was determined by thin-layer chromatography. Cholesterol esters were the only lipid that showed a marked increase in per cent distribution. A temporary change was that phospholipids decreased but triglycerides increased in the first week of infection. An explanation was given to this observation that polymorphonuclear leucocytes may have been the dominant cell population at this early stage of tuberculous lesions. As will be known from the succeeding paper, this cell type was as halt as the macrophage in the amount of total lipids and phospholipids, but twice as much in triglyceride content.<BR>A cell population whose main cell type was mycobacteria-ingesting macrophages was obtained from the tuberculous mouse lungs by glass-bead column method. Chemical analyses of this material comparing with normal and granulomatous lung homogenates suggested that the changes of lipid composition in the tuberculous lungs were the reflection of those in infected macrophages.
日本結核病学会の論文