定量法によるAbbott & Shanon法の再検討 (Shigella sonneiの産生するcolicinに関する研究-1-)

概要

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The quantitative method described by the authors in their previous report was used to examine the production of colicin from various type strains of Shigella sonnei, and the specificity and susceptibility of the indicator strains.The results obtained are summarized as follows.1. There were quantitative differences in the production of colicin as determined by Abbott and Shannon's method amoung the type strains used. A significant difference in the production of each colicin was observed in case a strain produced two different types of colicin.2. Some indicator strains used by Abbott and Shannon exhibited instability in their susceptibility to the same colicin produced by various strains to be typed, depending on the amount of colicin, or on the degree of the colicin activity.This may be one of the main causes for the occurrence of unstable patterns classified on the basis of Abbott and Shannon's typing. Poor reproducibility observed in certain cases may also be improved, if the bacterial counts of the indicator strains are adequate.
日本細菌学会の論文