デヒドロ酢酸分解細菌の分離とその代謝生成物について

概要

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Four kinds of bacteria, which metabolized dehydroacetic acid, were isolated from mid-air and soil using enrichment cultivation containing dehydroacetic acid in the medium as a sole source of carbon. Among those bacteria, two kinds of bacteria, C-5-1 from mid-air and M-1 from soil, were designated as Pseudomonas sp. C-5-1 and Pseudomonas sp. M-1, respectively. It was found that degradation of dehydroacetic acid by Pseudomonas sp. C-5-1 was more rapid than that of Pseudomonas sp. M-1. The metabolic pathway of dehydroacetic acid by both strains was assumed as follows: dehydroacetic acid→triacetic acid lactone→triacetic acid→acetic acid→TCA cycle. Furthermore, the accumulation of triacetic acid by Pseudomonas sp. M-1 was strongly affected by the presence of ammonium nitrate in culture medium, but with Pseudomonas sp. C-5-1 such an effect was not observed.
社団法人日本農芸化学会の論文