酵母Lipomyces starkeyiの脂質蓄積と細胞増殖を高める培地の検索

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A suitable medium was sought for controlling neutral lipid accumulation and cell proliferation of Lipomyces starkeyi. Deficiency of zinc sulfate and manganese chloride provided an increase in neutral lipid in cells (mg of neutral lipid/108 cells). The addition of large amounts of zinc sulfate, manganese chloride and potassium phosphate induced L. starkeyi to enhance cell proliferation (total cell number/ml) but limited neutral lipid accumulation. In medium consisting of low concentrations of zinc sulfate and manganese chloride, the neutral lipid in cells was approximately 22 times that of medium consisting of high concentrations of zinc sulfate, manganese chloride and potassium phosphate. The total cell number in medium for improving cell proliferation was approximately 20 times that of the medium for enhancing neutral lipid accumulation.
社団法人日本農芸化学会の論文