全モノアシルグリセリンの定量分析法に関する研究 : 合同実験結果について

概要

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For the purpose of establishing a standard method for the determination of total monoacylglycerol (MG), a collaborative study team has carried out analyses of specified samples by three methods ; Method I, II and III. In the Method I, perchloric acid was added simultaneously with periodic acid to a chloroform solution of a sample and then total MG was determined directly. In the Method II, 1-MG and 2-MG were equilibrated by addition of perchloric acid and then only 1-MG was determined by iodometry and the percentage of total MG was calculated with the use of the multiplication factor of 1.15. In the Method III, the amount of perchloric acid and the period of standing differed from them in the Meth d II. When saturated samples were analysed, intra- and interlaboratory scatterings of data obtained by each mothod were almost very small. The analytical values obtained by the Method I agreed very closely with the known values but the values by the Method II and III did not. With unsaturated samples, the analytical values obtained by the Method I, which gave satisfactory result for the saturated MG, were higher than the known values. Since the higher IV of the sample was, the higher the analytical value was, a part of periodic acid added was seemed to be consumed by double bonds present in the sample. It was concluded that with a saturated sample, the Method I was suitable for the determination of total MG.
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