Pyrimidine 5-nucleotidase 活性の日本人正常値および低濃度鉛汚染指標としての意義について

概要

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Normal enzymic activity levels of erythrocyte pyrimidine 5-nucleotidase were measured in 209 healthy adults. To determine its value as a biological index of the exposure to low level concentrations of lead, the dose-activity relationship between the lead concentration in the blood and the activity level of this enzyme was investigated with various substrate concentrations of CMP and the effects of other metal on pyrimidine 5-nucleotidase in the subjects with the various concentration of lead in blood. Two hundred and nine healthy adults, 109 male and 100 female subjects, 7 lead refinery workers and 3 clerks in a battery dissolution factory were used as subjects. The mean and standard deviation of pyrimidine 5-nucleotidase activity levels for the subjects were found to be 13.9±3.2 (μmoles Pi/gHb, hr). No significant difference was observed for sex or age, but regional differences were significant. A remarkably high negative correlation between logarithmic values of pyrimidine 5-nucleotidase activity and lead concentrations in the blood was observed. For the addition test in vitro, a 10-4M lead solution decreased the pyrimidine 5-nucleotidase activity level markedly, but effects of zinc on this enzyme were not observed.The above results suggest that erythrocyte pyrimidine 5-nucleotidase may be an index of subclinical lead exposure.
日本衛生学会の論文