Carbonic Anhydrase. lsozymes in Human Red Cells Immunological Determination and their Clinical Significances

概要

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Human erythrocyte carbonic anhydrase isozyme B and C were measured by a specific and immunological method. The levels of carbonic anhydrase B and C were determined in normal subjects, patients with several diseases. The levels of carbonic anhydrase B showed a decrease in hyperthyroidism and lead -exposed workers, and increased in patients with chronic obstructive lung diseases and patients with epilepsies under treatment with acetazolamide. Closely negative correlations were observed between carbonic anhydrase B and T3 Resin Uptake or Protein Bound Iodine levels in hyperthyroidism. Carbonic anhydrase C levels were not so much changed in those cases.<BR>Simultaneously, carbonic anhydrase B dependent esterase activity (active carbonic anhydrase B enzyme) was determined kinetically using the immunoadsorbent method in the abovepatients. The results were compared with the total enzyme protein (active and inactive carbonic anhydrase B enzyme), estimated by single radial immunodiffusion technique. In patients treated with acetazolamide, the specific activity of the carbonic anhydrase B (carbonic anhydrase B dependent esterase activity/total carbonic anhydrase B protein) decreased remarkably. Clinical significances of these isozymes in red cells were discussed.
Japan Society of Clinical Chemistryの論文