イオン交換樹脂によるアントラキノンスルホン酸異性体の分離,定量

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A salting-out chromatography has been applied to the separation of anthraquinone mono-and disulfonic acid isomers. In acidic sodium chloride solution, anthraquinone sulfonic acids are readily adsorbed on weakly acidic cation exchange resin (Amberlite CG-50). The distribution coefficient is increased with an increase of the NaCl coentration and with a decrease of pH value in the range of 1.0 to 5.0. In the separation of α- and β-anthraquinone sulfonic acids, the sample is placed on an Amberlite CG-50 (200400 mesh, bufferized at pH 2.7) column of 225 mm × 11mm φ, and the development is carried out with 0.1N NaCl of pH 2.7. The acids in effluent are determined by U. V. spectrophotometry. It was also possible to separate a mixture of a-anthraquinone sulfonic acid, 1, 5-, 1, 6-, 1.7- and 1, 8-anthraquinone disulfonic acids into each with a column of 270 mm × 17mm φ and 4N NaCl of pH 1.3 as a developer. The effects of the molecular structure of the chromatographed substance and the addition of organic solvent, such as methyl-, ethyl-, and n-propyl alcohol, on adsorption capacity are discussed.
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