Isolation and Anti-Fatty Liver Activity of a Novel Cerebroside from the Sea Cucumber Acaudina molpadioides
スポンサーリンク
概要
- 論文の詳細を見る
Cerebrosides are a kind of important bioactive substance in sea cucumber. A novel cerebroside, AMC-2, was purified from the less-polar lipid fraction of the sea cucumber Acaudina molpadioides by repeated column chromatography. The major structure of AMC-2 was analyzed by gas chromatography-mass spectra. The amide-linked fatty acid unit was confirmed to be four saturated and monounsaturated α-hydroxy fatty acids, the long-chain base was dihydroxy sphingoid base with one double bond, and the glycosyl group was glucose. We also investigated the anti-fatty liver activity of AMC-2 in rats with fatty liver induced by orotic acid. AMC-2 significantly reduced hepatic triglyceride (TG) and total cholesterol (TC) levels at a diet supplement of 0.03% and 0.006%. The indexes of stearoyl-CoA desaturase (SCD) activity and mRNA expression were significantly decreased by AMC-2. This indicates that AMC-2 ameliorated nonalcoholic fatty liver disease (NAFLD) through suppression of SCD activity and impaired the biosynthesis of monounsaturated fatty acids in the livers of the rats.
著者
-
Sugawara Tatsuya
Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University
-
XU Jie
College of Food Science and Engineering, Ocean University of China
-
WANG Yu-Ming
College of Food Science and Engineering, Ocean University of China
-
FENG Ting-Yu
College of Food Science and Engineering, Ocean University of China
-
ZHANG Bei
College of Food Science and Engineering, Ocean University of China
-
XUE Chang-Hu
College of Food Science and Engineering, Ocean University of China
関連論文
- Prevention of Aberrant Crypt Foci Formation by Dietary Maize and Yeast Cerebrosides in 1, 2-Dimethylhydrazine-treated Mice
- Rapid Quantitative Analysis of Sphingolipids in Seafood Using HPLC with Evaporative Light-Scattering Detection: Its Application in Tissue Distribution of Sphingolipids in Fish
- Analysis of Glucosylceramides from Various Sources by Liquid Chromatography-Ion Trap Mass Spectrometry
- Effects of reactive radicals and heat on trans-isomerization of eicosapentaenoic acid
- Isolation of Sphingoid Bases of Sea Cucumber Cerebrosides and Their Cytotoxicity against Human Colon Cancer Cells
- Characterization of Trans Eicosapentaenoic Acid Isomers: Oxidative Stability and Anti-Inflammatory Activity
- Efflux of Sphingoid Bases by P-Glycoprotein in Human Intestinal Caco-2 Cells
- Apoptosis Inducement by Plant and Fungus Sphingoid Bases in Human Colon Cancer Cells
- Antioxidative activities of a mycosporine-like amino acid, porphyra-334
- Inhibitory Effect of Topical Maize Glucosylceramide on Skin Photoaging in UVA-irradiated Hairless Mice