藻類におけるカロチノイドの比較生化学-II : モツレミルのカロチノイドとそれらの生合成

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Besides α-carotene, β-carotene, siphonaxanthin and neoxanthin, the existence of ε-carotene, lutein, zeaxanthin, tataxanthin, prolycopene like compound, phytofluene like compound, and phytoene like compound was confirmed as the constituents of carotenoids in Codium intricatum. To study the biosynthesis of carotenoid in Codium, 2-C14 -mevalonic acid was injected to the plant. After incubation for 48 hours in sea water, the carotenoids were extracted, and xanthophyll fraction was separated from carotene one by partition coefficient and each of them was submitted to thin layer chromatograyhy. After development, a sheet of X-ray film (Fuji X-ray film, medical) was placed on contact with the thin layer chromatoplate, subsequent development of the film showed the black band on the film corresponding to the exact shape and location of each compound on the thin layer chromatogram. The result showed that 2-C14-mevalonic acid was incorporated into siphonaxanthin, neoxanthin, phytoene like compound and phytofluene like compound, therefore, it was assumed that the carotenoids in Codium would be synthesized in the almost same way as in land plants.
公益社団法人日本水産学会の論文