Production of New Type of Antisera to Thyrotropin Releasing Hormone (TRH) Using Carbodiimide Coupling and an Application to Immunohistochemistry

スポンサーリンク

概要

• 論文の詳細を見る

• A new type of antibody was successfully produced in rabbits with TRH-BSA as an antigen. The conjugation was based on the amide bond using a carbodiimide coupling method. With this antiserum an enzyme immunoassay (EIA) for TRH was developed, and the neutralization of anti-TRH activity with an excess amount of synthetic TRH was confirmed. Very little or no cross-reaction was observed with TRH-BDB-BSA, an immunogen for generating anti-TRH serum used in previous reports. When the new type of antibodies were used for immunohistochemistry in the mammalian hypothalamus, many neuronal somata with TRH immunoreactivity were visible in animals without colchicine treatment. In addition to the known distribution of TRH cell bodies in the hypothalamus, another cell group was demonstrated to contain TRH immunoreactivity. The anti-TRH serum produced by this method should be useful for investigating extrahypothalamic TRH systems.

著者

• Ochi Junzo

  Department Of Anatomy (director: Prof. Y. Sano) Kyoto Prefectural University Of Medicine

• INANAGA Kazutoyo

  Department of Nernropsychiatry and Institute of Brain Diseases, Kurume University School of Medicine

• Yamamoto Toshiharu

  Department Of Anatomy Shiga University Of Medical Science

• Shirouzu Muneyasu

  Department Of Anatomy Shiga University Of Medical Science

• Kimura Hiroshi

  Department Of Anatomy Ii Shiga University Of Medical Science

• KIMURA Hiroshi

  Department of Anatomy, Shiga University of Medical Science

• INANAGA KAZUTOYO

  Department of Psychiatry, Kurume University School of Medicine

関連論文

• A New Aspect of the Carotid Body Function Controlling Hypoxic Ventilatory Decline in Humans
• The scid Mutation Does Not Affect Slowly Repairing Potentially Lethal Damage That Is Sensitive To 0.23 M NaCl.
• Mental Stress Reduces Coronary Flow Velocity Reserve
• Membrane Localization of Protein-Tyrosine Phosphatase 1B is Essential for its Activation of Sterol Regulatory Element-Binding Protein-1 Gene Expression and Consequent Hypertriglyceridaemia
• A MODIFIED ZINC IODIDE-OSMIUM TETROXIDE STAINING METHOD FOR NERVE TERMINALS IN SPINAL CORDS OF THE RAT
• Classification of Synaptic Terminals by Means of ZIO Method
• A Modification of ZIO Method for Synaptic Endings
• Properties of Monolithic Silica Columns for HPLC
• THE ORIGIN AND THE EVOLUTION OF THE THIOESTER-CONTAINING PROTEIN(Physiology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• FUNCTIONAL INVESTIGATION OF THE DRP/DHP/CRAM/UNC-33 FAMILY IN PLANARIANS, DUGESIA JAPONICA(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• Possibility of Preventive Treatment for EBV-associated NK Cell-lineage Proliferative Disorders
• RESULTS OF MULTIPLE CORONARY ARTERY BYPASS GRAFTING IN JAPANESE PATIENTS
• CORONARY ARTERY BYPASS GRAFTING BY UTILIZING THE INTERNAL MAMMARY ARTERY GRAFT IN 100 JAPANESE PATIENTS
• Immunohistochemical and in situ Hybridization Studies on Endothelin in the Rat Eye
• Trial of intraventricular ribavirin therapy for subacute sclerosing panencephalitis in Japan
• Primary Cerebral Angiitis Containing Marked Xanthoma Cells With Massive Intraparenchymal Involvement : Case Report
• Drug-Induced Hypersensitivity Syndrome due to Mexiletine Associated with Human Herpes Virus 6 and Cytomegalovirus Reactivation
• Postnatal development of Serotonergic terminal in rat cerebral cortex
• Ontogenesis of 5HT immunoreactive neurons in the rat brain
• Oral Vitamin C restores impaired Coronary Flow Reserve in Smokers
• Circadian Variation in Coronary Flow Reserve is disappeared in Coronary Heart Disease
• CD4- and CD56-positive T-cell line MTA, established from natural killer-like T-cell leukemia/lymphoma
• 32. On the Experience of the Brachial Vertebral Angiography
• 29. A surgical case of an enormous acoustic tumor with atypical symtoms.
• 72. Experimental studies of hypothermia from the neurosurgical point of view. IV.
• 70. Experimental studies of hypothermia from the neurosurgical point of view. II.
• Neurocysticercosis as Solitary Parenchymal Lesion Confirmed by Mitochondrial Deoxyribonucleic Acid Sequence Analysis : Case Report
• Synthesis of Optically Active Polyarylates Having Axially Chiral Binaphthylene Unit
• Biosynthesis of Poly(3-hydroxyalkanoate)s with Terminal Alkene Groups in the Side Chains by Chromobacterium sp.
• Biosynthesis of Poly(3-hydroxyalkanoate)s with Terminal Alkene Groups in the Side Chains by Chromobacterium sp.
• Production of Polyester Blend of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and Poly(3-hydroxyalkanoate) with Saturated and Unsaturated Monomers from 4-Hydroxybutyric Acid by Chromobacterium sp.
• Synthesis and Biodegradation of Polyesters Based on O - Methylene Tartaric Acid
• Effective Biosynthesis of Poly(3-hydroxybutyrate) from Plant Oils by Chromobacterium sp.
• Synthesis and Photoinduced Transformation of a Helical Aromatic Polyamide Having Optically Active Binaphthol Units in the Main Chain
• Production and Characterization of Poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) from L-Valine by Ralstonia eutropha
• Biosynthesis of Terpolythioesters with 3-Mercaptopropionate Unit
• Purificaiton and characterization of Secretory-Type GDP-L-Fucose: β-D-Galactoside 2-α-L-Fucosyltransferase from Human Gastric Muscosa
• Hemiconvulsion-hemiplegia syndrome and primary human herpesvirus 7 infection
• Early changes in bone mineral content caused by acute irradiation are not observed after daily fractionation: A study dual-energy X-ray absorptiometry
• Postmortem computed tomography findings as evidence of traffic accident-related fatal injury
• Successful Treatment of Neonatal Herpes Simplex-Type 1 Infection Complicated by Hemophagocytic Lymphohistiocytosis and Acute Liver Failure
• Multiplex real-time PCR for the simultaneous detection of herpes simplex virus, human herpesvirus 6, and human herpesvirus 7
• FIBRONECTIN-LIKE IMMUNOREACTIVE STRUCTURES IN EMBRYONIC RAT BRAIN (Modern Histo- and Cytochemistry in Neuroscience : Embryogenesis and Regeneration of the Nervous System)
• Immunohistochemical Demonstration of Fibronectin in the Rat Embryo.
• FLUOROHISTOCHEMISTRY AND ELECTRON MICROSCOPY OF THE CHROMAFFIN CELLS IN THE ADRENAL GLAND OF THE CLAWED TOAD, XENOPUS LAEVIS
• Microspectrofluorometry of Formaldehyde= Induced Fluorescence : Automatic Correction of Emission Spectrum and the Spectrum of DA and 5-HT Mixture
• Distribution of Brain Stem Serotonergic Neurons with Projection to Cingulum and Spinal Lateral Funiculus of the Rat
• Cerebrospinal Fluid (CSF)-Contacting, Monoamine-Containing Neurons of the Lamprey. A Comparative Fluorescence and Electron Microscope Study on the Same Section
• Cerebrospinal Fluid-Contacting Neurons in the Third Ventricular Wall of the Bullfrog: Distribution of Monoamine-Containing Cells and Acetylcholinesterase-Positive Cells
• Fluorescence and Electron Microscopic Observation on Chromaffin Cells in the Adrenal and Periadrenal Tissue of Xenopus laevis
• Adrenalin-Storing, Noradrenalin-Storing and Small Granule Chromaffin Cells in the Bullfrog Adrenal. A Comparative Fluorescence and Electron Microscopy on the Identical Section.
• Microspectrofluorometric Differentiation among Fluorophores of Dopamine, Noradrenaline and Adrenaline in Model Droplets and in Tissue Sections
• An Improved Microspectrofluorometer with Epi-illumination for Ultraviolet Excitation
• MONOAMINE-CONTAINING CELLS IN THE GASTRIC MUCOSA OF THE KITTEN: A NEW METHOD EMPLOYING COMPARATIVE FLUORESCENCE AND ELECTRON MICROSCOPY ON IDENTICAL CELLS
• Endothelin A receptor-like immunoreactivity on the basal infoldings of rat renal tubules and collecting ducts
• Extensive Distribution of Galanin-Immunoreactive Neuronal Systems in a Fresh Water Snail, Indoplanorbis exustus, and Partial Coexistence with Choline Acetyltransferase-like Immunoreactivity
• Fluorescence and Electron Microscope Studies on Liquor-contacting Subependymal Cells of the Frog Preoptic Area
• Immunocytochemical demonstration of serotonin (5HT) in the human platelet.
• A STUDY OF POTASSIUM PERMANGANATE FIXATION FOR ELECTRON MICROSCOPIC DETECTION OF SMALL GRANULAR VESICLES IN THE SYMPATHETIC NERVOUS SYSTEM
• Taurine-like Immunoreactive Structures in the Mouse Liver
• Immunohistochemical demonstration of 6-HT-containing neurons in the CNS.
• Imunohistochemical Demonstration of Fibronectin in Rat Brains Lesioned with 5,6-dihydroxytryptamine (5,6DHT).
• Endothelin-Induced c-fos Expression in the Rat Forebrain
• EFFECTS OF ID-540 ON AVERAGED PHOTOPALPEBRAL REFLEX IN MAN
• Production of New Type of Antisera to Thyrotropin Releasing Hormone (TRH) Using Carbodiimide Coupling and an Application to Immunohistochemistry
• Fluorescence and Electron Microscopy of Catecholamines : A trial for electron microscopical differentiation between dopamine and noradrenaline (Histochemistry of Bioactive Amines)
• Phylogenetic Study of Catecholamine Nerves in the Telencephalon Using a Supersensitive Histofluorescence Technique
• A New Technique for Immunohistochemistry Using Hybrid IgG. : I. Procedure of Hybrid IgG Production.
• Production of new type antisera to thyrotropin releasing hormone (TRH) using carbodiimide coupling and the application to immunohistochemistry.
• SILVER IMPREGNATION AND FLUORESCENCE HISTOCHEMISTRY OF THE SUBEPENDYMAL CELLS IN THE PREOPTIC RECESS OF THE BULLFROG : Rana Catesbeiana
• Fluorescent Retrograde Labeling of Serotonergic (5HT) Neurons in the Central Nervous System (CNS) of the Rat
• A Study of KMnO_4 Fixation for Granulated Vesicles
• Endothelin B Receptor-like Immunoreactivity in Podocytes of the Rat Kidney
• Calcitonin gene-related peptide-like nervous system in the optic lobe and peduncle complex of the octopus, Octopus vulgaris
• Hypothermic, but not normothermic, ischemia causes a drastic increase in cyclooxygenase-2 immunoreactive granule cells in rat dentate gyrus after 4 hours of ischemic reperfusion
• ENDOTHELIN A RECEPTOR-LIKE IMMUNOREACTIVITY ON THE BASOLATERAL CYTOPLASMIC MEMBRANE OF THE BASAL INFOLDING OF RENAL TUBULES AND COLLECTING DUCTS(Biochemistry & Endocrinology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Jap
• Endothelin 1-Like Immunoreactivity in the Hypothalamo-Hypophysial System of the Bullfrog, Rana catesbeiana
• Galanin-Containing Nerve Nets in Hydra (Hydra japonica) and Freshwater Medusa (Craspedacusta sowerbyi)
• STUDY ON THE CORRELATION BETWEEN CLINICAL AND PHYSIOLOGICAL RESPONSES TO CHLORPROMAZINE
• Thyrotropin releasing hormone and analogues rapidly lose their TRH receptor binding capacities in solution. A discrepancy from degradation.:A discrepancy from degradation
• Hypothermia and gastric lesions in rats exposed to immobilization stress.
• Hyperthermia induced by thyrotropin-releasing hormone (TRH, protirelin) in the rat.
• Haloperidol and biperiden plasma levels in a pregnant atypical psychotic woman and a neonate - A case report.:A Case Report
• Binding of TRH, DN -1417 and MK-771 to TRH Receptors in the Rat Brain:Relationship to their central actions
• Effects of aniracetam, a nootropic drug, in senile dementia. A preliminary report.:A Preliminary Report
• Synergism between methamphetamine and alcohol in a case of methamphetamine psychosis.
• Effects of thyrotropin-releasing hormone in schizophrenia.
• Simultaneous determination of dopamine, serotonin, 3,4-dihydroxyphenyl-acetic acid, homovanillic acid, 3-methoxytyramine and 5-hydroxyindole-3-acetic acid by high performance liquid chromatography with electrochemical detection.
• A Thyrotropin Releasing Hormone Analog, DN -1417, Increases Endogenous Dopamine Release from Slices of Rat Striatum and Nucleus Accumbens
• ELECTROPHYSIOLOGICAL STUDIES ON HALLUCINATION
• The chronic effect of nomifensine on rat stereotyped behavior.
• Effectiveness of baclofen in schizophrenia.
• Effects of five .BETA.-LPH-neuropeptides and DN-1417, a derivative of TRH, on grip test performance in rats: Possible neuroleptic action.
• Effects of a TRH Analog (DN-1417) and TRH-T on the Electroconvulsive Threshold
• Benzodiazepines and the Gamma-aminobutyric Acid (GABA) Receptor
• Effects of a TRH analog (DN-1417) on hippocampal pyramidal neurons in vitro.
• Effects of neuroleptics on dopamine release from striatal slices.
• The Effect of a TRH Analog on Pyramidal Neurons in the Guinea-pig Hippocampus in vitro
• Phase I Study on Mianserin Hydrochloride (Org GB 94):Safety and Tolerance
• THREE CASES OF SCHIZOPHRENIA TREATED WITH L-DOPA

もっと見る 閉じる

スポンサーリンク