A fluorimetric and enzymatic determination for the estimation of total bile acid sulfates in urine.

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A fluorimetric and enzymatic determination for the estimation of total bile acid sulfates in urine is described. The sulfate fraction was obtained from a urine specimen by passing through a Sep-pak C<SUB>18</SUB> cartridge, followed by group separation by ion-exchange chromatography on a lipophilic gel, piperidinohydroxypropyl Sephdex LH-20.<BR>The obtained sulfate fraction was solvolized by the modified method of Galeazzi et al and deconjugated bile acids were estimated with fluorimetrc and enzymatic method.<BR>Overall recovery of glycine- and taurine-conjugated bile acid 3-sulfates from normal urine ranged from 90.5 to 93.7% and uncojugated bile acid 3-sulfates from 48.7 to 78.0%. The range of normal values for total bile acid sulfates in urine was found to be 2.6-17.5μmoles/l (mean: 10.4±4.9) for males and 1.6-15.3μmoles/l (mean: 6.5±4.9) for females.