Localization of Estrogen Receptor-.BETA. in Rat Tissue by Immunohistochemistry.

概要

論文の詳細を見る
Steroid hormones regulate cell functions via specific receptors. We have raised a polyclonal antiserum using a peptide specific for estrogen receptor-β in order to determine the cellular sites of expression of the receptor. In the 6 week old rat, estrogen receptor-β was localized to the cell nuclei in both male and female reproductive organs, such as the ventral, lateral and dorsal prostate, seminal vesicle, coagulating and ampullary gland, spermatic duct, testis, uterus and ovary. In the ventral and lateral prostate, estrogen receptor-β was present in glandular epithelial cells and strong staining was observed. In the dorsal prostate, seminal vesicle, coagulating gland, ampullary gland and uterus, epithelial cells were immunopositive and moderate staining was observed. In the spermatic duct, estrogen receptor-β positive nuclei were observed in the epithelial cells. In the stromal cells of many tissue, estrogen receptor-β positive nuclei were also observed.In the testis, nuclei of Sertoli cells were immunopositive. In the ovary, estrogen receptor-β was present in the follicles and corpus luteum and strong staining was observed. Thus immunohistochemistry was shown to be an important investigative tool for visualising specific sites of expression of estrogen receptor-β in rat male and female reproductive organs.
北関東医学会の論文