Flow cytometric analysis of Kupffer cell phagocytic function in rats.

概要

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New method of measurement of Kupffer cell phagocytic function in vivo by using flow cytometery (FCM) was investigated. After injection of Flurescence isothiocyanate (FITC)-latex beads into penial vein, rats were sacrificed at selected times to isolate Kupffer cells by collagenase digestion-Percoll gravity gradient centrifusion method. The reaction time increased gradually and reached a plateau at 1 hour after injection of FITC-latex beads. The most suitable concentration of FITC-latex beads solution for FCM analysis was 6×10<SUP>10</SUP>beads/rat. The average of FCM-phagocytic rate (FCM-PR) in normal rats was 51.4%. Most Kupffer cells phagocytized 2 beads. In reticuloendothelial system (RES) enhanced rats pretreated with OK-432, FCM-PR increased to 74.3% significantly. And also in RES depressed rats pretreated with methyl palmitate, FCM-PR was suppressed to 18.1% significantly. The correlation coefficient between FCM-PR and phagocytic index which was measured by carbon clearance method was 0.89.
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