遊離肝細胞移植の研究--脾臓内移植肝細胞の電顕学的研究

概要

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Hepatocellular transplantation into the spleen utilizing isolated hepatocytes has been investigated as a new attempt to compensate for impaired liver function. Present study was undertaken to evaluate ultrastructural alterations in hepatocytes transplanted into the splenic parenchyma up to 6 weeks following transplantation.<BR>Light microscopical studies revealed viable hepatocellular islets in the splenic parenchyma, although minimal cytoplasmic changes were observed. Electron microscopical studies demonstrated moderate changes in organelles, which proceeded to develop gradually after transplantation. However, distortion and fragmentation of membranes of cytoplasm and organelles were not observed. Moreover, newly formed bile canaliculi and tight junctions which indicate reconstitution of hepatic trabeculae were observed between adjacent cell membranes.<BR>The transplanted hepatocytes preserved their morphological characteristics with fine structures as hepatocytes up to 6 weeks. Our present sutdies suggest that inoculated hepatocytes maintain their hepatocellular functions after transplantation on the basis of the persistence of cellular viability.
社団法人日本肝臓学会の論文