ビブリオ病経口ワクチンによるアユの免疫機構の研究-1-体表における感染防御作用

概要

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Both orally immunized ayu, Plecoglossus altivelis, and control fish were challenged with organism, Vibrio anguillarum, by three ways. First, fish were exposed to organisms discharged from naturally infected fish for 24 hours. Organisms were isolated at high percentage from almost all part of body in the control fish, while the isolation rate was low particularly on the body surface in the immunized fish. Secondary, fish were bathed in bacterial suspension of a concentration of 10<SUP>7</SUP> cells per milliliter for 15 minutes. Twenty four hours after challenge organisms were isolated from the skin of the control fish, then the number of organism increased gradually for the next 72 hours. No organism was detected from the intestine and contents of it in both groups or the skin in the immunized group. Finally, fish were injected intramuscularly resulting almost equal mortality in both groups. Agglutinin titer in the body surface mucus rose to 1: 64 in the immunized group, but did not in the control group. Agglutinin titer in neither sera nor intestinal mucus rose in both groups. The body surface mucus of the immunized fish prevented organisms from adhering to the skin more effectively than that of the control fish. From these results it is assumed that the defense effect by the oral immunization is attributed mainly to the agglutinin secreted in the body surface.
日本魚病学会の論文