Effect of anti-CD4, anti-OKDR antibody and γ-interferon on cluster formation by human peripheral blood dendritic cells and T cells

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Dendritic cells (DC) were successfully isolated from human peripheral blood and investigated for cluster formation by co-culturing them with autologous T cells at 37°C.Cluster formation was recognized in the presense of anti-CD4, anti-OKDR antibody and γ-interferon (IFN). However, the proliferative response of clusters as measured by 3H-thymidine incorporation was depressed in a dose-dependent manner in the presense of anti-CD4 and anti-OKDR antibody. On the other hand, non-clustered cells showed slight 3H-thymidine incorporation in the presense or absense of anti-CD4 and anti-OKDR antibody. The proliferative response of clusters was augmented by pretreating DC with interleukin 1 (IL-1) and depressed by pretreatment with γ-IFN.These results suggested that IL-1 has a stimulatory effect on the proliferative reponse of the cluster while anti-CD4, anti-OKDR antibody and γ-IFN have an inhibitory effect, even though these factors exhibited no effect on cluster formation.
日本臨床免疫学会の論文