EFFECTS OF NEUTRAL SALTS ON HEPATIC MICROSOMAL DRUG-METABOLIZING ENZYME SYSTEM IN RATS
スポンサーリンク
概要
- 論文の詳細を見る
Effects of neutral salts on the drug-metabolizing enzyme system were measured in hepatic microsomes of rats in vitro. Aminopyrine N-demethylation was markedly enhanced by Li<SUB>2</SUB>SO<SUB>4</SUB>, Na<SUB>2</SUB>SO<SUB>4</SUB>, and K<SUB>2</SUB>SO<SUB>4</SUB>. Salts such as LiCi, NaCl, and KCI caused an enhancement of the demethylation following by an inhibition at high concentrations. KBr, KI, and KSCN always inhibited the demethylation. Aniline hydroxylation, on the contrary, was not stimulated by the sulfates, and all other salts inhibited the hydroxylation with increasing concentration. The effectiveness of the neutral salts on changing aminopyrine or aniline oxidation activity followed Hofmeisters lyotropic series of ions: SCN<SUP>-</SUP>>I<SUP>-</SUP> Br<SUP>-</SUP>>Cl<SUP>-</SUP>>SO<SUB>4</SUB><SUP>--</SUP> as anions and Li<SUP>+</SUP>>Na<SUP>+</SUP>, K<SUP>+</SUP> as cations. KSCN, KI and KBr caused both the conversion of cytochrome P-450 to cytochrome P-420 and the inhibition of NADPH cytochrome <I>c</I> reductase activity; however, all other salts used in these experiments showed no change of those components. Enhancement of aminopyrine N-demethylation by the sulfates was reversible. It was concluded that cytochrome P-450 associated with aminopyrine N-demethylation is different from that of aniline hydroxylation in the hydrophobic environment of microsomes, and sulfate or chloride causes an enhancement of only cytochrome P-450 activity associated with the demethylation.
- 社団法人 日本薬理学会の論文
著者
-
Aniya Yoko
Laboratory of Molecular Genetics and Pharmacology, School of Health Sciences, Faculty of Medicine, U
-
Aniya Yoko
Laboratory Of Functional And Molecular Pharmacology Graduate School Of Medicine University Of The Ry
-
MATSUSAKI Kichihiko
Miyazaki Medical College
関連論文
- Modulation of Membrane-Bound Glutathione Transferase Activity by Phospholipids Including Cardiolipin
- Medicinal Herb, Thonningia Sanguinea Protects Against Aflatoxin B_1 Acute Hepatotoxicity in Fischerl 344 Rats.
- Reactive nitrogen species derived activation of rat liver microsomal glutathione S-transferase (DRUG METABOLISM) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
- Purification of Liver Serine Protease Which Activates Microsomal Glutathione S-Transferase : Possible Involvement of Hepsin(Biochemistry)
- Free Radical Scavenging and Hepatoprotective Actions of the Medicinal Herb, Crassocephalum crepidioides from the Okinawa Islands(Biochemistry/Molecular Biology)
- Antioxidant and Hepatoprotective Actions of the Medicinal Herb Artemisia campestris from the Okinawa Islands
- P-16 Inhibitory effects of the medicinal herb, Thonningia sanguinea on liver drug metabolizing enzymes of rats.(Proceedings of the 27th Annual Meeting)
- Activation of Hepatic Microsomal Glutathione S-Transferase of Rats by a Glutathione Depletor, Diethylmaleate
- Activation of Rat Liver Microsomal Glutathione Transferase by Hepsin
- Activation of Liver Microsomal Glutathione S-Transferase Activity by Heating
- Preferential Proteolysis and Activation of Oxidatively Modified Liver Microsomal Glutathione S-Transferase of Rat
- ENHANCEMENT OF HEPATIC MICROSOMAL ANILINE HYDROXYLATION BY HABU SNAKE (Trimeresurus flavoviridis) VENOM FRACTIONS
- Antioxidative Action of the β-Adrenoceptor Antagonist Bopindolol and Its Metabolite 18-502
- EFFECTS OF NEUTRAL SALTS ON HEPATIC MICROSOMAL DRUG-METABOLIZING ENZYME SYSTEM IN RATS
- Interferon-β confers on mouse macrophages a resistance to glucocorticoid suppression of inducible nitric-oxide synthase mRNA expression
- EFFECT OF THE SPINE VENOM FROM THE CROWN-OF-THORNS STARFISH, ACANTHASTER PLANCI, ON DRUG-METABOLIZING ENZYME IN RAT LIVER
- Protective Effect of the Mold Monascus Anka Against Acetaminophen-Induced Liver Toxicity in Rats
- Evaluation of Nitric Oxide Formation from Nitrates in Pig Coronary Arteries
- Inhibitory Effects of Alpinia speciosa K. SCHUM on the Porphyrin Photooxidative Reaction
- Effects of 2-Nicotinamidoethyl Nitrate (SG-75, Nicorandil) on Indomethacin-Induced Contractions of Isolated Dog Coronary Arteries
- Effect of antioxidants in Okinawan medicinal herbs on aldose reductase activity.(GENERAL SESSION BY POSTER PRESENTATION)(ACTIVE OXYGEN ETC)
- SMOOTH MUSCLE CONTRACTILE ACTION OF THE VENOM FROM THE CROWN-OF-THORNS STARFISH, ACANTHASTER PLANCI
- ENHANCEMENT OF AMINOPYRINE N-DEMETHYLATION BY SULFATE IN LIVER MICROSOMES OF RATS
- Heat Stable Protein with Anticoagulant and Smooth Muscle Contractile Actions Isolated from Habu (Trimeresurus flavoviridis) Venom
- Glutathione S-transferases and chloroform toxicity in streptozotocin-induced diabetic rats.
- Activation of Microsomal Glutathione S-Transferase in tert-Butyl Hydroperoxide-Induced Oxidative Stress of Isol-ated Rat Liver.
- Alteration of Liver Glutathione S-Transferase and Protease Activities by Cobalt Chloride Treatment of Rats.
- Antioxidative Action of the Nitrovasodilator Nicorandil: Inhibition of Oxidative Activation of Liver Microsomal Glutathione S-Transferase and Lipid Peroxidation.