Activation of Liver Microsomal Glutathione S-Transferase Activity by Heating
スポンサーリンク
概要
- 論文の詳細を見る
The effect of heating on rat liver microsomal glutathione S-transferase activity was investigated. The microsomal glutathione S-transferase activity increased with an elevation of temperature and reached a maximal level at 50℃ for 30 min, at which point the enhancement was 2.6-fold. The microsomal glutathione S-transferase activity had one low K_m value for 1-chloro-2,4-dinitrobenzene at room temperature. However, two K_m values were observed in heated microsomes. N-Ethylmaleimide increased microsomal glutathione S-transferase activity 7.7-fold and a 2.5-fold increase in the activity was observed even after heating of microsomes at 55℃ for 10 min. On the other hand, there was no additional activation in the heated-microsomal glutathione S-transferase by glutathione disulfide or diamide with glutathione. The increased activity of the microsomal glutathione S-transferase by heating was not reversed by dithiothreitol. These results indicate that the microsomal glutathione S-transferase activity is activated by heating through a mechanism different from the activation caused by sulfhydryl reagents.
- 公益社団法人日本薬学会の論文
著者
-
Aniya Yoko
Laboratory of Molecular Genetics and Pharmacology, School of Health Sciences, Faculty of Medicine, U
-
Aniya Yoko
Laboratory Of Functional And Molecular Pharmacology Graduate School Of Medicine University Of The Ry
-
Aniya Yoko
Laboratory Of Physiology And Pharmacology School Of Health Sciences University Of The Ryukyus
関連論文
- Modulation of Membrane-Bound Glutathione Transferase Activity by Phospholipids Including Cardiolipin
- Medicinal Herb, Thonningia Sanguinea Protects Against Aflatoxin B_1 Acute Hepatotoxicity in Fischerl 344 Rats.
- Reactive nitrogen species derived activation of rat liver microsomal glutathione S-transferase (DRUG METABOLISM) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
- Purification of Liver Serine Protease Which Activates Microsomal Glutathione S-Transferase : Possible Involvement of Hepsin(Biochemistry)
- Free Radical Scavenging and Hepatoprotective Actions of the Medicinal Herb, Crassocephalum crepidioides from the Okinawa Islands(Biochemistry/Molecular Biology)
- Antioxidant and Hepatoprotective Actions of the Medicinal Herb Artemisia campestris from the Okinawa Islands
- P-16 Inhibitory effects of the medicinal herb, Thonningia sanguinea on liver drug metabolizing enzymes of rats.(Proceedings of the 27th Annual Meeting)
- Activation of Hepatic Microsomal Glutathione S-Transferase of Rats by a Glutathione Depletor, Diethylmaleate
- Activation of Rat Liver Microsomal Glutathione Transferase by Hepsin
- Activation of Liver Microsomal Glutathione S-Transferase Activity by Heating
- Preferential Proteolysis and Activation of Oxidatively Modified Liver Microsomal Glutathione S-Transferase of Rat
- ENHANCEMENT OF HEPATIC MICROSOMAL ANILINE HYDROXYLATION BY HABU SNAKE (Trimeresurus flavoviridis) VENOM FRACTIONS
- Antioxidative Action of the β-Adrenoceptor Antagonist Bopindolol and Its Metabolite 18-502
- EFFECTS OF NEUTRAL SALTS ON HEPATIC MICROSOMAL DRUG-METABOLIZING ENZYME SYSTEM IN RATS
- Interferon-β confers on mouse macrophages a resistance to glucocorticoid suppression of inducible nitric-oxide synthase mRNA expression
- EFFECT OF THE SPINE VENOM FROM THE CROWN-OF-THORNS STARFISH, ACANTHASTER PLANCI, ON DRUG-METABOLIZING ENZYME IN RAT LIVER
- Protective Effect of the Mold Monascus Anka Against Acetaminophen-Induced Liver Toxicity in Rats
- Evaluation of Nitric Oxide Formation from Nitrates in Pig Coronary Arteries
- Inhibitory Effects of Alpinia speciosa K. SCHUM on the Porphyrin Photooxidative Reaction
- Effects of 2-Nicotinamidoethyl Nitrate (SG-75, Nicorandil) on Indomethacin-Induced Contractions of Isolated Dog Coronary Arteries
- Effect of antioxidants in Okinawan medicinal herbs on aldose reductase activity.(GENERAL SESSION BY POSTER PRESENTATION)(ACTIVE OXYGEN ETC)
- SMOOTH MUSCLE CONTRACTILE ACTION OF THE VENOM FROM THE CROWN-OF-THORNS STARFISH, ACANTHASTER PLANCI
- ENHANCEMENT OF AMINOPYRINE N-DEMETHYLATION BY SULFATE IN LIVER MICROSOMES OF RATS
- Heat Stable Protein with Anticoagulant and Smooth Muscle Contractile Actions Isolated from Habu (Trimeresurus flavoviridis) Venom
- Glutathione S-transferases and chloroform toxicity in streptozotocin-induced diabetic rats.
- Activation of Microsomal Glutathione S-Transferase in tert-Butyl Hydroperoxide-Induced Oxidative Stress of Isol-ated Rat Liver.
- Alteration of Liver Glutathione S-Transferase and Protease Activities by Cobalt Chloride Treatment of Rats.
- Antioxidative Action of the Nitrovasodilator Nicorandil: Inhibition of Oxidative Activation of Liver Microsomal Glutathione S-Transferase and Lipid Peroxidation.