Purification of Liver Serine Protease Which Activates Microsomal Glutathione S-Transferase : Possible Involvement of Hepsin(Biochemistry)
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概要
- 論文の詳細を見る
Rat liver microsomal glutathione S-transferase (MGST1) is known to be activated by trypsin, however, it has not been clarified whether MGST1 is activated by a protease present in liver. In the present study we purified the MGST1 activating protease from liver microsomes and finally identified that the protease is hepsin, a type II transmembrane serine protease. When the protease was incubated with the purified MGST1 or liposomal MGST1 at 4℃, MGST1 activity was increased 3-4.5 fold after 3-6d. In electrophoretic and immunoblot analyses after the incubation of MGST1 with the protease MGST1 dimer and its degraded fragment were detected. These results suggest that the rat liver microsomal hepsin functions as MGST1 activating/degrading enzyme.
- 公益社団法人日本薬学会の論文
- 2006-05-01
著者
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Aniya Yoko
Laboratory of Molecular Genetics and Pharmacology, School of Health Sciences, Faculty of Medicine, U
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Nakama Shinji
Laboratory Of Functional And Molecular Pharmacology Graduate School Of Medicine University Of The Ry
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Aniya Yoko
Laboratory Of Functional And Molecular Pharmacology Graduate School Of Medicine University Of The Ry
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Kunii Daisuke
Laboratory Of Functional And Molecular Pharmacology Graduate School Of Medicine University Of The Ry
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Sato Izumi
Laboratory Of Functional And Molecular Pharmacology Graduate School Of Medicine University Of The Ry
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Shimoji Miyuki
Laboratory Of Functional And Molecular Pharmacology Graduate School Of Medicine University Of The Ry
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Yamazaki Kazuko
Laboratory Of Functional And Molecular Pharmacology Graduate School Of Medicine University Of The Ry
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IKEBE Masashi
Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the
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HACHIMAN Teruyuki
Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the
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TAMAKI Atsuko
Laboratory of Functional and Molecular Pharmacology, Graduate School of Medicine, University of the
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Ikebe Masashi
Laboratory Of Functional And Molecular Pharmacology Graduate School Of Medicine University Of The Ry
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Tamaki Atsuko
Laboratory Of Functional And Molecular Pharmacology Graduate School Of Medicine University Of The Ry
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Hachiman Teruyuki
Laboratory Of Functional And Molecular Pharmacology Graduate School Of Medicine University Of The Ry
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