Pharmacological study on Ba2+-stimulated catecholamine secretion from cultured bovine adrenal chromaffin cells: Possible relation of Ba2+ action to Ca2+-activated secretory mechanism.
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概要
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The stimulatory action of Ba<SUP>2+</SUP> on catecholamine secretion from cultured bovine adrenal chromaffin cells was studied to elucidate a possible relationship between Ba<SUP>2+</SUP> action and the Ca<SUP>2+</SUP>-mediated secretory mechanism. Catecholamine secretion was dramatically stimulated by Ba<SUP>2+</SUP> in the absence of external Ca<SUP>2+</SUP>, and this stimulatory action was observed in a concentration-dependent manner. Ba<SUP>2+</SUP> evoked the concomitant release of dopamine β-hydroxylase in a similar manner to the Ca<SUP>2+</SUP>-dependent secretion. The stimulation of catecholamine secretion by low concentrations of Ba<SUP>2+</SUP> was markedly inhibited by protein kinase inhibitors, polymyxin B and trifluoperazine (TFP). The inhibitory action of polymyxin B, but not that of TFP, on the Ba<SUP>2+</SUP> action was attenuated by elevating the concentration of Ba<SUP>2+</SUP> in the incubation mixture. The stimulatory action of Ba<SUP>2+</SUP> was enhanced by a protein kinase C activator, 12-O-tetradecanoylphorbol 13-acetate (TPA). In contrast to the acute effect of TPA, chronic exposure of chromaffin cells to high concentration of TPA reduced catecholamine secretion stimulated by Ba<SUP>2+</SUP> as well as high K<SUP>+</SUP> and carbamylcholine. These findings suggest the possibility that Ba<SUP>2+</SUP> may activate Ca<SUP>2+</SUP>-mediated secretory processes presumably through its action on protein kinase C, thus resulting in the stimulation of catecholamine secretion from bovine adrenal chromaffin cells
- 公益社団法人 日本薬理学会の論文
著者
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Hamano Shuichi
Department of Pathological Science and Technology, The Institute of Health Bioscience, The Universit
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Morita Kyoji
Department Of Pharmacology Tokushima University School Of Medicine
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OKA Motoo
Department of Pharmacology II, Osaka University School of Medicine
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Azuma Mami
Department of Pharmacology, Tokushima University School of Medicine
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Teraoka Kazuhiko
Department of Pharmacology, Tokushima University School of Medicine
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