ELEVATION OF STEROID 5α-REDUCTASE mRNA LEVELS IN RAT CEREBELLUM BY TOLUENE INHALATION : POSSIBLE RELATION TO GFAP EXPRESSION
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概要
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Toluene, a commonly used industrial solvent, is known to be toxic to both neuronal and glial cells, and has been shown to increase the immunoreactivity of glial fibrillary acidic protein(GFAP)in the brain. However, the mechanism of toluene-induced GFAP expression is poorly understood. Recently, GFAP mRNA expression in cultured astrocytes has been shown to be modulated by various steroid hormones, such as progesterone, testosterone, and their 5α-reduced metabolites. Therefore, it seems possible that steroid hormones may play a potential role in the enhancement of GFAP expression observed following toluene exposure. To address this possibility, the effect of toluene inhalation on the expression of mRNAs encoding GFAP and steroidogenic enzymes in rat brain was examined. Toluene exposure increased GFAP protein contents without any significant alteration in GFAP mRNA levels in the hippocampus. In contrast, the elevation of both GFAP protein contents and its mRNA levels was observed in the cerebellum following toluene exposure. Further studies indicated that toluene exposure increased steroid 5α-reductase(5α-R)mRNA levels prior to the elevation of GFAP mRNA in the cerebellum, whereas neither 5α-R nor GFAP mRNA levels in the hippocampus were significantly affected by toluene exposure. These results suggest that toluene inhalation may enhance GFAP gene expression in the rat cerebellum, and propose the possibility that the elevation of 5α-R expression, and hence 5α-reduced metabolites of steroid hormones, is presumably related to toluene-induced GFAP mRNA expression.
- 日本トキシコロジー学会の論文
- 2000-08-17
著者
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KUBO Shin-ichi
Department of Legal Medicine, School of Medicine, The University of Tokushima
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Kubo Shin-ichi
Department Of Forensic Medicine Institute Of Health Biosciences The University Of Tokushima Graduate
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TOKUNAGA Itsuo
Department of Forensic Medicine, Institute of Health Biosciences, The University of Tokushima Gradua
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GOTOHDA Takako
Department of Forensic Medicine, Institute of Health Biosciences, The University of Tokushima Gradua
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KUWADA Aki
Department of Pharmacology, Tokushima University School of Medicine
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MORITA Kyoji
Department of Pharmacology, Tokushima University School of Medicine
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Morita K
Department Of Pharmacology Tokushima University School Of Medicine
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Kubo Shuji
Department Of Forensic Medicine Institute Of Health Biosciences The University Of Tokushima Graduate
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Kuwada Aki
Department Of Pharmacology Tokushima University School Of Medicine
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Morita Kyoji
Department Of Pharmacology Tokushima University School Of Medicine
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Gotohda Takako
Department Of Forensic Medicine Institute Of Health Biosciences The University Of Tokushima Graduate
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Tokunaga I
Department Of Forensic Medicine Institute Of Health Biosciences The University Of Tokushima Graduate
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Tokunaga Itsuo
Department Of Forensic Medicine The University Of Tokushima Graduate School
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