Ca2+ Inactivation of Voltage-Dependent Na+ Channels in Culturec Bovine Adrenal Chromaffin Cells: Further Studies on Inhibition of Veratridine-Induced Catecholamine Secretion by External Ca2+.
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概要
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The stimulatory actions of veratridine (VTD) on catecholamine secretion and Na<SUP>+</SUP> influx in cultured bovine adrenal chromaffin cells were studied in the presence of high concentrations of Ca<SUP>2+</SUP> in the incubation mixture. Catecholamine secretion evoked by VTD was reduced by elevating the external Ca<SUP>2+</SUP> concentration to higher than 2 mM. Under the same conditions, VTD-stimulated <SUP>22</SUP>Na<SUP>+</SUP> uptake into the cells was also reduced by elevating the extracellular Ca<SUP>2+</SUP> concentration. In contrast, the secretory action of VTD was not significantly suppressed by preloading Ca</SUP>2+</SUP> to the cells. Furthermore, the effects of extracellular Ca</SUP>2+</SUP> on the sensitivities of the cells to VTD and tetrodotoxin (TTX) were examined, and neither their sensitivities to VTD nor those to TTX were shown to be significantly altered by elevating the Ca</SUP>2+</SUP> concentration in the incubation mixture. These results seem to indicate that the elevation of extracellular Ca</SUP>2+</SUP> concentration may cause the inhibition of VTD-induced catecholamine secretion as a consequence of the inactivation of voltage-dependent Na</SUP>+</SUP> channels, and suggest that Ca</SUP>2+</SUP> may directly act on the cell surface, and the site of Ca</SUP>2+</SUP> action is presumably distinct from the sites of both VTD and TTX actions in the plasma membranes of adrenal chromaffin cells.
- 公益社団法人 日本薬理学会の論文
著者
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Hamano Shuichi
Department of Pathological Science and Technology, The Institute of Health Bioscience, The Universit
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OKA Motoo
Department of Pharmacology II, Osaka University School of Medicine
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Oka Motoo
Department of Pharmacology, Tokushima University School of Medicine
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Azuma Mami
Department of Pharmacology, Tokushima University School of Medicine
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Morita Kyoji
Nancy Pritzker Laboratory of Developmental and Molecular Neurobiology, Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine
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