Radioallergosorbent Test(RAST)によるスギ花粉症の検討 : 1.スギ花粉症におけるRASTの基礎的検討

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Although the concentration of the japanese cedar specific IgE antibody in blood can be measured with RAST, the Phadebas RAST Kit contains no japanese cedar allergen disc. Japanese cedar allergen disc (I : 20) was newly prepared. It was evaluated whether this japanese cedar allergen disc was practical or not in the RAST of Japanese Cedar Pollinosis. Serum samples werecollected from 86 patients with Japanese Cedar Pollinosis and 10 normal adults. RAST was performed with Phadebas reagents and (1 :20) japanese cedar allergen disc according to Phadebas experimental instructions. Results were as follow: 1) Phadebas, mountain cedar allergen discs did not react with the japanese cedar specific IgE in the sera of Japanese Cedar Pollinosis. 2) Japanese cedar allergen was well coupled to paper discs and japanese cedar allergen discs (1 : 20) absorbed so well the japanese cedar specific IgE antibody that RAST with this japanese cedar allergen disc can be carried out without the modification of Phadebas experimental directions. 3) The results of RAST with this japanese cedar allergen disc should be expressed following new criteria other than Phadebas RAST criteria. According to the RAST results of normal persons, negative RAST, doubtful RAST and positive RAST were newly classified instead of Phadebas RAST class. 4) The RAST results of 86 patients suffering from Japanese Cedar Pollinosis were 4 cases of negative RAST. 13 cases of doubtful RAST and 69 cases of positive RAST.
社団法人日本耳鼻咽喉科学会の論文

Radioallergosorbent Test(RAST)によるスギ花粉症の検討 : 1.スギ花粉症におけるRASTの基礎的検討

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