Plaque Formation Assay for Human Parainfluenza Virus Type 1
スポンサーリンク
概要
- 論文の詳細を見る
Human parainfluenza virus type 1 (hPIV1) generally does not show visible plaques in common cell lines, including Lewis lung carcinoma-monkey kidney (LLC-MK2) cells, by plaque formation assays for human parainfluenza virus type 3 (hPIV3) and Sendai virus. In several conditions of the plaque formation assay, complete elimination of serum proteins in the overlay medium was necessary for visualization of hPIV1-induced plaque formation in LLC-MK2 cells. We developed a plaque formation assay for hPIV1 isolation and titration in LLC-MK2 cells using an initial overlay medium of bovine serum albumin-free Eagles minimum essential medium containing agarose and acetylated trypsin for 4—6 d followed by a second overlay staining medium containing agarose and neutral red. The assay allowed both laboratory and clinical hPIV1 strains to form large plaques. The plaque reduction assay was also performed with rabbit anti-hPIV1 antibody as a general evaluation model of viral inhibitors to decrease both the plaque number and size. The results indicate that the plaque formation assay is useful for hPIV1 isolation, titration, evaluation of antiviral reagents and epidemiologic research.
- 公益社団法人 日本薬学会の論文
著者
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TAKAHASHI Tadanobu
Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Core Research
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SUZUKI Takashi
Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Core Research
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Takahashi N
Department Of Biochemistry University Of Shizuoka School Of Pharmaceutical Sciences:core Research Fo
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Suzuki Takashi
Department Of Biochemistry University Of Shizuoka School Of Pharmaceutical Sciences:core Research Fo
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Takahashi Tadanobu
Department Of Biochemistry School Of Pharmaceutical Sciences University Of Shizuoka And Global Coe P
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Fukushima Keijo
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation
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Takaguchi Masahiro
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation
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Ueyama Hiroo
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation
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Ito Seigo
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation
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Kurebayashi Yuuki
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation
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Kawanishi Tomohiro
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation
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McKimm-Breschkin Jennifer
CSIRO Molecular and Health Technologies
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Takimoto Toru
Department of Microbiology and Immunology, University of Rochester Medical Center
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Minami Akira
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation
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Takimoto Toru
Department Of Hygiene And Microbiology Faculty Of Veterinary Medicine Hokkaido University
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Ueyama Hiroo
Department Of Biochemistry University Of Shizuoka School Of Pharmaceutical Sciences And Global Coe Program For Innovation In Human Health Sciences
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Suzuki Takashi
Department Of Anatomic Pathology Tohoku University School Of Medicine
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Takaguchi Masahiro
Department Of Biochemistry University Of Shizuoka School Of Pharmaceutical Sciences And Global Coe Program For Innovation In Human Health Sciences
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Minami Akira
Department Of Biochemistry School Of Pharmaceutical Sciences University Of Shizuoka And Global Coe Program
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Fukushima Keijo
Department Of Biochemistry University Of Shizuoka School Of Pharmaceutical Sciences And Global Coe Program For Innovation In Human Health Sciences
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Ueyama Hiroo
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation in Human Health Sciences, University of Shizuoka
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Kawanishi Tomohiro
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation in Human Health Sciences, University of Shizuoka
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Ito Seigo
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation in Human Health Sciences, University of Shizuoka
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Kurebayashi Yuuki
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation in Human Health Sciences, University of Shizuoka
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Fukushima Keijo
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation in Human Health Sciences, University of Shizuoka
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Takahashi Tadanobu
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation in Human Health Sciences, University of Shizuoka
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Minami Akira
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation in Human Health Sciences, University of Shizuoka
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Takaguchi Masahiro
Department of Biochemistry, School of Pharmaceutical Sciences and Global COE Program for Innovation in Human Health Sciences, University of Shizuoka
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