ビーバー・ラムの製造試験-3-キリングにおけるシスチンおよびシステインの消長
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For the purpose to make clear chemical, physical and structural changes of wool fiber in killing process known as a pretreatment for dyeing beaver lamb, in this paper, as the first step, the effects of killing process on cystine and cysteine contents of wool were investigated.Ammonia, sodium carbonate and sodium hydroxide were used as killing agents and temperature, concentration and time of treatment were also varied in the experiment. The length of float was 1: 25. After killing, 200mg of wool was hydrolyzed with 30% sulphuric acid, then cystine and cysteine contents of the wool were determined colorimetrically by reacting with Folin reagent.The results obtained were as follows.1. The digestion of wool cystine in the killing was high during the first thirty minutes of treatment, especially in the case with ammonia and sodium carbonate more than fifty per cent of wool cystine were digested.2. In the killing with ammonia and sodium carbonate, the effect of temperature on the digestion of wool cystine was more remarkable than that of increasing concentration. In the killing with sodium hydroxide, temperature also influenced cystine digestion greatly, but effect of concentration was greater than in the killing with ammonia and sodium carbonate.3. In the killing with ammonia and sodium carbonate, the amount of cystine digested at 20°C and 40°C was extremely low at higher concentration than about 0.5N, but the amount of of cystine digested at 60°C increased linearly with the increase of concentration.4. The feature of cystine digestion in the killing with sodium hydroxide was different from that in the cases with ammonia and sodium carbonate, and the amounts of cystine digested at 20°C increased proportionally with concentration.5.The amount of cystine digested at 40°C in the killing with ammonia was the same as the amount at 20°C in the killing with sodium carbonate. The amount of cystine digested at 40°C with sodium carbonate was also the same as the amount at 20°C with sodium hydroxide.6. As to cysteine contents of wool, the contents became lower than those of untreated wool when the killing was done at 40°C and 60°C with sodium hydroxide. All other treated wool in this experiment showed higher contents of cysteine than those of untreated wool.
- 社団法人 日本畜産学会の論文
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