牛乳中の血清アルブミンおよび免疫グロブリンGの免疫学的定量

概要

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Single radial immunodiffusion technique was employed for quantitation of the serum albumin (BSA) and IgG immunoglobulin in bovine milk. Two kinds of antisera, anti-BSA and anti-IgG, were prepared by immunizing rabbits with the corresponding antigens plus Freunds complete adjuvant.It was confirmed that these antisera reacted only with the corresponding antigens, and did not crossreacted with α-lactalbumin, β-lactoglobulin and the other proteins in milk.When 4μl of an antigen solution of an unknown concentration is allowed to diffuse from a well in a thin layer of agar gel containing the antiserum in the ratio of 2.5 per cent of its original concentration at 37°C for 72 hours, the square of diameter of final precipitate ring is directly proportional to the amaount of antigen employed. By using this system, the amount of BSA ranged from 6.25-100mg/dl and IgG ranged from 12.5-300mg/dl in milk were measured directly without any pretreatment of samples. The cofficient of variation of the measurements to an amount of antigen was less than 2.5 per cent.It was also shown that the average BSA contents in normal milk was 14.28mg/dl and that of IgG was 73.44mg/dl, and the mastitic milk contained much more BSA and IgG as compared with normal milk.
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