Immunopotentiator from Pantoea agglomerans 1 (IP-PA1)は経口投与によりメラノーマ担癌マウスの生存を改善する
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概要
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To investigate the usefulness of the immunopotentiator from Pantoea agglomerans 1 (IP-PA1) as a supportive drug in melanoma therapy, we analyzed the immunological effects of IP-PA1 on melanoma-inoculated model mice. Oral administration of IP-PA1 increased the serum levels of tumor necrosis factor (TNF)-α at 2 h after the administration and interferon (IFN)-γ and IL-12 at 12 h after the administration in naïve BALB/cCrSlc mice as evaluated by ELISA. IP-PA1 did not affect the proliferation of melanoma cells directly determined by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Combinatory treatment of IP-PA1 with doxorubicin for 9 days increased the serum levels of IFN-γ and IL-12 by 71.0 and 15.3%, respectively, compared to the treatment of doxorubicin alone in melanoma-bearing C57BL/6NCrSlc mice as evaluated by ELISA. It also increased the proportion of natural killer (NK) cells and the ratio of CD4+ to CD8+ T cells in the spleen from 6.1 ± 0.3 to 7.4 ± 0.5% and from 1.25 ± 0.03 to 1.38 ± 0.04, respectively, compared to the treatment of doxorubicin alone as analyzed by flow cytometry. The mean survival period of melanoma-bearing, doxorubicin treated mice was prolonged from 31.4 ± 7.1 to 35.3 ± 8.4, 51.1 ± 5.4, and 45.0 ± 8.4 days by combinatory treatment of IP-PA1 at the daily doses of 0.1, 0.5, and 1 mg/kg, respectively. In conclusion, the results of the present study suggest the usefulness of IP-PA1 as a supportive drug in melanoma therapy.
著者
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WATANABE Gen
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Toky
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TAYA Kazuyoshi
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Toky
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Taya Kazuyoshi
Laboratory Of Veterinary Physiology Department Of Veterinary Medicine Faculty Of Agriculture Tokyo U
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松本 安喜
東京大学 農生命科研究
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SOMA Gen-Ichiro
Institute for Health Sciences, Tokushima Bunri University
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HAYASHI Yoshihiro
Laboratory of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The
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MATSUMOTO Yasunobu
Laboratory of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The
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Watanabe Gen
Laboratory Of Veterinary Physiology Department Of Veterinary Medicine Faculty Of Agriculture Tokyo U
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KOHCHI Chie
Institute for Health and Sciences, Tokushima Bunri University
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Soma Gen-ichiro
Institute For Health And Sciences Tokushima Bunri University
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Kohchi Chie
Institute For Health And Sciences Tokushima Bunri University
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Hirota Yoshikazu
Laboratory Of Animal Microbiology College Of Agriculture
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Hirota Yoshikazu
Laboratory Of Aminal Microbiology College Of Agriculture
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HEBISHIMA Takehisa
Laboratories of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, To
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