インヒビン受動免疫法によるミニシバヤギの排卵数増加
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概要
- 論文の詳細を見る
We reported previously that passive immunization against inhibin enhances follicular growth and increases the ovulation rate. However, the ovulation rate was not comparable to the number of follicles. Therefore, the aim of this study was to attempt to increase the ovulation rate by increasing the interval between inhibin immunization and PGF2α injection. Five miniature Shiba goats were treated with 10 ml inhibin antiserum (inhibin-AS) developed against [Tyro30]-inhibin α (1-30). A control group (n=5) was treated with normal goat serum. All animals were injected intramuscularly with 125 μg PGF2α 72 h after treatment to induce estrus and ovulation. Blood samples were collected for hormonal assay and the ovulation rate was determined by laparotomy. In contrast to the control group, there was a significant increase in plasma concentrations of FSH in the immunized group. After luteolysis, plasma concentrations of estradiol-17β increased markedly to a preovulatory peak about 2 folds higher (P<0.01) than that of controls. In addition, the ovulation rate was greater in the immunized group (14.4 ± 2.2) than in the control group (2.2 ± 0.6), and the mean number of follicles ≥ 4 mm in diameter was 10.0 ± 0.8 in the inhibin-AS group compared with 2.4 ± 0.3 in control group. The present results demonstrate that immunoneutralization of endogenous inhibin increased FSH secretions in miniature shiba goats. The increased FSH secretion enhanced follicular growth and increased the ovulation rate. Additionally, increasing the interval between inhibin-AS and PGF2α injections (to 72 h) resulted in a greater ovulation rate compared with the previous protocol (48 h). Therefore, inhibin-AS treatment proved to be an effective alternative to exogenous gonadotropin methods for induction of superovulation in goats.
- 日本繁殖生物学会の論文
- 2004-12-01
著者
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WATANABE Gen
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Toky
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TAYA Kazuyoshi
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Toky
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MEDAN Mohamed
Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology
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Taya Kazuyoshi
Laboratory Of Veterinary Physiology Department Of Veterinary Medicine Faculty Of Agriculture Tokyo U
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Taya K
Laboratory Of Veterinary Physiology Department Of Veterinary Medicine Tokyo University Of Agricultur
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Watanabe Gen
Laboratory Of Veterinary Physiology Department Of Veterinary Medicine Faculty Of Agriculture Tokyo U
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Medan Mohamed
Faculty Of Veterinary Medicine Suez Canal University
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名倉 義夫
Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology
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金澤 浩子
National Livestock Breeding Center, Nagano Station
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KANAZAWA Hiroko
National Livestock Breeding Center, Nagano Station
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FUJITA Masaru
National Livestock Breeding Center, Nagano Station
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Medan Mohamed
東京農工大学 農学部獣医学科獣医生理学研究室
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Fujita Masaru
National Livestock Breeding Center Nagano Station
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NAGURA Yoshio
National Livestock Breeding Center, Nagano Station
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Takimoto Kazuhiro
Department Of Veterinary Science National Institute Of Health
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Kanazawa Hiroko
National Livestock Breeding Center
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Nagura Yoshio
National Livestock Breeding Center
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Fujita Masaru
National Livestock Breeding Center
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Medan Mohamed
Laboratory Of Veterinary Physiology Tokyo University Of Agriculture And Technology
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Medan Mohamed
エジプト
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Watanabe Gen
Laboratories Of Veterinary Physiology Dep. Of Veterinary Medicine Fac. Of Agriculture Tokyo Univ. Of
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