インヒビン中和法による幼若ラットの過排卵誘起
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概要
- 論文の詳細を見る
The effects of passive immunoneutralization of endogenous inhibin on ovulation rate in immature rats were investigated. Efficiency of superovulation on production of fertilized oocytes was compared between the inhibin antiserum (inhibin-AS) and equine chorionic gonadotropin (eCG) protocols. Immature female Wistar strain rats were superovulated with a single injection of 100-200 μl inhibin-AS, with and without an injection of human chorionic gonadotrophin (hCG). A total of 77.8% of the 26-30-day-old rats treated with a single injection of 100-200 μl inhibin-AS ovulated 72 h after treatment, while rats given normal goat serum (NGS; 200 μl) did not ovulate. At 28 days of age, all of the inhibin-AS treated rats ovulated when additional hCG treatment was given, whereas the number of ovulated oocytes was not affected. The number of ovulated oocytes in the inhibin-AS-hCG treated groups was significantly higher than that of the NGS-hCG treated group. In addition, plasma concentrations of FSH in the inhibin-AS-hCG treated group significantly increased compared with the NGS treated group. While the percentage of mated rats in the 200 μl inhibin-AS-hCG treated group was significantly lower than that of the 15 IU eCG-hCG treated group, the fertilization rate was comparable between the two groups. The number of fertilized oocytes in the 200 μl inhibin-AS-hCG treated group was significantly higher in comparison with the 15 IU eCG-hCG treated group. These results suggest that immunoneutralization of endogenous inhibin could be a reliable method for induction of superovulation to collect a large number of normally fertilized oocytes in immature rats.
- 日本繁殖生物学会の論文
著者
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WATANABE Gen
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Toky
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TAYA Kazuyoshi
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Toky
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ARAI Koji
Department of Pediatrics, Hirosaki University School of Medicine
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MEDAN Mohamed
Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology
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Taya Kazuyoshi
Laboratory Of Veterinary Physiology Department Of Veterinary Medicine Faculty Of Agriculture Tokyo U
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Arai Koji
Department Of Pediatrics Hirosaki University School Of Medicine
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Watanabe Gen
Laboratory Of Veterinary Physiology Department Of Veterinary Medicine Faculty Of Agriculture Tokyo U
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石亀 晴道
Laboratory of Veterinary Physiology, Faculty of Agriculture, Tokyo University of Agriculture and Tec
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川口 真似子
Department of Toxicology and Parmacology, Faculty of Pharmacy, Musashino University
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FUKUDA Junji
Laboratory of Veterinary Physiology, Faculty of Agriculture, Tokyo University of Agriculture and Tec
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ISHIGAME Harumichi
Laboratory of Veterinary Physiology, Faculty of Agriculture, Tokyo University of Agriculture and Tec
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KAWAGUCHI Mariko
Department of Toxicology and Parmacology, Faculty of Pharmacy, Musashino University
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Ishigame Harumichi
Laboratory Of Veterinary Physiology Tokyo University Of Agriculture And Technology
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Fukuda Atsushi
Laboratory Of Veterinary Physiology Tokyo University Of Agriculture And Technology
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Medan Mohamed
Laboratory Of Veterinary Physiology Tokyo University Of Agriculture And Technology
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Watanabe Gen
Laboratories Of Veterinary Physiology Dep. Of Veterinary Medicine Fac. Of Agriculture Tokyo Univ. Of
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TAYA Kazuyoshi
Laboratory of Veterinary Physiology, Faculty of Agriculture, Tokyo University of Agriculture and Technology
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MEDAN Mohamed
Laboratory of Veterinary Physiology, Faculty of Agriculture, Tokyo University of Agriculture and Technology
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