Establishment of the PCR System Specific to Salmonella spp. and Its Application for the Inspection of Food and Fecal Samples.

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概要

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• We established the PCR detection system specific to Salmonella species using Salmonella enterotoxin gene (stn). The detection limit was one bacterial cell per one gram of fecal and minced-meat samples using enrichment procedure by Tripticase soy broth or Salmonella enrichment broth, respectively. We concluded that this PCR system is useful for the practical application in the field of the public hygiene.

• 公益社団法人 日本獣医学会の論文

著者

• HAYASHI Hideo

  Deparment of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University

• Cheun Hyeng-il

  Department Of Veterinary Microbiology Obihiro University Of Agriculture And Veterinary Medicine

• Makino Sou-ichi

  Department Of Veterinary Microbiology Obihiro University Of Agriculture And Veterinary Medicine

• Kurazono Hisao

  Department Of Microbiology Institute Of Basic Medical Sciences University Of Tsukuba

• Shirahata Toshikazu

  Department Of Applied Veterinary Science

• Suzuki Saori

  Department Of Environmental And Life Sciences Toyohashi University Of Technology

• SHIRAHATA Toshikazu

  Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan

• CHONGSANGUAM Manas

  Department of Microbiology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki 305-8557, Japan

• SUZUKI Saori

  Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan

• MAKINO Sou-ichi

  Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan

• KURAZONO Hisao

  Department of Medical Technology, School of Health Sciences, Okayama University, Okayama 700-8558, Japan

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