RNA Interference With Small Hairpin RNAs Transcribed From a Human U6 Promoter-Driven DNA Vector
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概要
- 論文の詳細を見る
RNA interference (RNAi), a process of sequence-specific gene suppression, has been known as a natural gene regulatory mechanism in a wide range of organisms. Recently, a small-interference RNA (siRNA) technology has been reported to produce post-transcriptional gene silencing in mammalian cells. In the present study, we constructed a human U6 promoter-driven mammalian expression vector to produce hairpin double-stranded RNA and transfected this into a human cell line. Using this siRNA system, we were able to knock down the gene expression of an enhanced green fluorescence protein. This result indicates that the plasmid vector-based siRNA system is a promising method to downregulate gene expression in human cells.
- 社団法人 日本薬理学会の論文
- 2003-10-01
著者
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Koike Hiroko
Department Of Cell Biology Institute Of Nephrology Graduate School Of Medical And Dental Sciences Ni
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Koike Hiroko
Department Of Psychiatry University Of Illinois At Chicago
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SUGAYA Kiminobu
Department of Psychiatry, University of Illinois
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Kwak Young-don
Department Of Psychiatry University Of Illinois At Chicago
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Sugaya Kiminobu
Department Of Psychiatry The Psychiatric Institute University Of Illinois At Chicago
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KOIKE Hiroko
Department of Biology, College of Liberal Arts, Saitama University
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SUGAYA Kiminobu
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Science University of Tokyo
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