カリフラワ-DNAポリメラ-ゼの分離とその性質〔英文〕
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概要
- 論文の詳細を見る
Two distinct DNA polymerases, designated A and B, have been partially purified from rapidly growing apical tissues of the cauliflower inflorescence (Brassica oleracea, var. botrytis). They were readily separable from each other, because enzyme A was adsorbable on an anion-exchanger, but enzyme B was not. The effects of divalent rations and ionic strength on both enzyme activities were very different. Enzyme A utilized activated DNA well at low concentration of MgCl2 or at low ionic strength, but heat-denatured DNA was utilized more effectively than was activated DNA at high concentration of MgCl2. Enzyme B also utilized much more heat-denatured DNA and poly (rA)•(dT)10 at high ionic strength. Gel chromatography with Sephadex G-200 revealed that enzyme A has a higher molecular weight (approx. 100, 000) than enzyme B (approx. 75, 000). It has been also described that some points of properties in enzyme A and B resemble to those of the two DNA polymerase -α and -β from mammalian cells, respectively. The existence of the third enzyme, designated C, in the fraction with enzyme A was suggested with an assay system using poly (rA)•(dT)10 as the template.
- 日本遺伝学会の論文
著者
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Matsumoto Hiroyuki
Department of Electrical Engineering, Toyo University
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Fukasawa Hirosuke
Department Of Biology Faculty Of Science Kobe University
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Yamaguchi Masamitsu
Department Of Applied Biology Faculty Of Textile Sciences Kyoto Institute Of Technology
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CHOU MEI-YIN
Department of Biology, Faculty of Science, Kobe University
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Matsumoto Hiroyuki
Department Of Biochemistry And Molecular Biology University Of Oklahoma Health Sciences Center
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Matsumoto Hiroyuki
Department Of Biochemistry And Molecular Biology The University Of Oklahoma Health Sciences Center
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YAMAGUCHI MASAMITSU
Department of Biology, Faculty of Science, Kobe University
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山口 政光
Department of Biology, Faculty of Science, Kobe University
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