Presence of Associated Exonuclease in DNA Polymerase-B from Cauliflower Inflorescence
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概要
- 論文の詳細を見る
Two DNA polymerases, A and B, isolated from cauliflower (Brassica oleracea, var. botrytis) were assayed for their deoxyribonuclease activities, using ^3H-labeled DNA synthesized in vitro by cauliflower DNA polymerase-A. Nuclease activities were coeluted with the activities of DNA polymerase-A and -B by DNA-cellulose and phospho-cellulose column chromatography and in the case of polymerase-B, this occurred even after treatment with subtilisin. Profiles of thermal inactivation, sensitivity to N-ethylmaleimide and inhibition by subtilisin of the nuclease activity resembled those of the polymerase activity in polymerase-B, but not those of polymerase-A. These observations suggest that at least DNA polymerase-B possesses an associated deoxyribonuclease activity. This nuclease exhibited a specificity for degradation of DNA in the 3'→5' direction.
- 日本植物生理学会の論文
著者
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Matsumoto Hiroyuki
Department of Electrical Engineering, Toyo University
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Fukata Hideki
Department Of Bioenvironmental Medicine Graduate School Of Medicine Chiba University
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Fukata Hideki
Department Of Biology Faculty Of Science Kobe University
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Fukasawa Hirosuke
Department Of Biology Faculty Of Science Kobe University
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Matsumoto Hiroyuki
Department Of Biochemistry And Molecular Biology University Of Oklahoma Health Sciences Center
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Matsumoto Hiroyuki
Department Of Biology Faculty Of Science Kobe University
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Matsumoto Hiroyuki
Department Of Biochemistry And Molecular Biology The University Of Oklahoma Health Sciences Center
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