雄ゲッチンゲンミニブタ精巣におけるステロイドホルモン合成酵素P450scc,P450c17,3βHSDおよびP450aromの免疫局在
スポンサーリンク
概要
- 論文の詳細を見る
The objective of this study was to investigate immunolocalization of steroidogenic enzymes in Göttingen miniature (GM) pig testes. Testes of 6 adult GM pigs were obtained in September 1996 (n=2), February (n=2) and June (n=2), 1997. Steroidogenic enzymes were immunolocalized using polyclonal antisera raised against bovine adrenal cholesterol side-chain cleavage cytochrome P450 (P450scc), human placental 3β-hydroxysteroid dehydrogenase (3βHSD), porcine testicular 17α-hydroxylase cytochrome P450 (P450c17), and human placental aromatase cytochrome P450 (P450arom). Histologically, all types of spermatogenic cells including mature-phase spermatozoa in seminiferous tubules were observed in all testes throughout the year. Moreover, P450scc, 3 βHSD, P450c17and P450arom were identified in Leydig cells but not in Sertoli cells of all testes. These results suggested that adult GM pig testes have the ability to produce germ cells throughout the year, and the synthesis of progestin, androgen and estrogen occurs in the Leydig cells of GM pig testes.
- 日本繁殖生物学会の論文
著者
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TAYA Kazuyoshi
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Toky
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渡辺 元
食品薬品安全センター秦野研究所
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MEDAN Mohamed
Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology
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TANIOKA YOSHIKUNI
Central Institute for Experimental Animals
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Taya Kazuyoshi
Laboratory Of Veterinary Physiology Department Of Veterinary Medicine Faculty Of Agriculture Tokyo U
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Tsubota Toshio
United Graduate School Of Veterinary Medicine Gifu University
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Watanabe Gen
Laboratory Of Veterinary Physiology Department Of Veterinary Medicine Faculty Of Agriculture Tokyo U
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翁 強
Faculty of Biological Science and Technology, Beijing Forestry University
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渡辺 元
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Toky
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WENG Qiang
Faculty of Biological Science and Technology, Beijing Forestry University
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渡辺 元
Laboratory Of Veterinary Physiology Faculty Of Agriculture Tokyo University Of Agriculture And Techn
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Medan Mohamed
Laboratory Of Veterinary Physiology Tokyo University Of Agriculture And Technology
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Watanabe Gen
Laboratories Of Veterinary Physiology Dep. Of Veterinary Medicine Fac. Of Agriculture Tokyo Univ. Of
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MEDAN Mohamed
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
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