A Physiology Study of Escherichia coli Overexpressing Phosphoenolpyruvate Carboxykinase
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概要
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To determine whether intracellular ATP levels can be affected, Escherichia coli overexpressing phosphoenolpyruvate carboxykinase (pck) or phosphoenolpyruvate carboxylase (ppc) were grown in glucose minimal medium. The Pck-overexpressing cells showed approximately twice the intracellular ATP concentration, with 22% slower growth than the Ppc-overexpressing strain. This unexpected result of higher ATP coupled with slower growth is discussed based on transcriptome analysis.
著者
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LEE Sang
Department of Pulmonary and Critical Care Medicine, Gachon University, Gil Medical Center
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Kwon Yeong-Deok
Department of Life Science, Catholic University of Korea
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Kim Pil
Department of Biotechnology, Catholic University of Korea
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Kwon Yeong-deok
Department Of Biotechnology Catholic University Of Korea:(present Office)biotech R & D Center Pa
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Kim Pil
Department Of Biotechnology Catholic University Of Korea
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