Extracellular signal-regulated kinase (ERK) inhibition attenuates cigarette smoke extract (CSE) induced-death inducing signaling complex (DISC) formation in human lung fibroblasts (MRC-5) cells
スポンサーリンク
概要
- 論文の詳細を見る
Cigarette smoke (CS), a major risk factor in emphysema, causes cell death by incompletely understood mechanisms. Death-inducing signaling complex (DISC) formation is an initial event in Fas-mediated apoptosis. We demonstrated cigarette smoke extract (CSE) induced DISC formation in human lung fibroblasts (MRC-5). The aim of this study was to investigate the involvement of extracellular signal-regulated kinase (ERK) MAPK activation in CSE induced DISC formation. Immunoprecipitaion (IP) for Fas and Western Immunoblot (IB) analysis for caspase 8 were then performed to show DISC. Lactate dehydrogenase (LDH) release was measured using a cytotoxicity detection kit. MTT assay was used as a measure of cell viability. We demonstrated that CSE induces DISC formation in MRC-5 using IP for Fas and IB for caspase 8. ERK was expressed in MRC-5 exposed to CSE. MEK-1 inhibitor (PD98059) decreased DISC formation in MRC-5 exposed to 20% CSE at 1 hr, and cell viability, as assessed by colorimetric MTT assay, was increased in MEK-1 inhibitor treated MRC-5 cells after 24 hr CSE exposure compared to the control. Inhibiting ERK significantly decreased the caspase-3,-8 activity in MEK-1 inhibitor treated MRC-5 cells compared to the control.The DISC formation, initial event of extrinsic apoptotic pathway, is a primary component of CSE- induced death in MRC-5, and ERK activation plays an active role in the DISC formation and downstream pathway. These results suggest that modulation of ERK may have therapeutic potential in the prevention of smoke-related lung injury.
- 2010-02-01
著者
-
PARK Jeong-Woong
Department of Pulmonary and Critical Care Medicine, Gachon University, Gil Medical Center
-
YOON Jin
Department of Pulmonary and Critical Care Medicine, Gachon University, Gil Medical Center
-
KIM Yu
Department of Pulmonary and Critical Care Medicine, Gachon University, Gil Medical Center
-
KYUNG Sun
Department of Pulmonary and Critical Care Medicine, Gachon University, Gil Medical Center
-
LEE Sang
Department of Pulmonary and Critical Care Medicine, Gachon University, Gil Medical Center
-
JEONG Sung
Department of Pulmonary and Critical Care Medicine, Gachon University, Gil Medical Center
-
MOON Chanil
Department of Cardiology, Gachon University, Gil Medical Center
-
Moon Chanil
Department Of Cardiology Gachon University Gil Medical Center
-
Yoon Jin
Department Of Pulmonary And Critical Care Medicine Gachon University Gil Medical Center
-
Jeong Sung
Department Of Pulmonary And Critical Care Medicine Gachon University Gil Medical Center
-
Kyung Sun
Department Of Pulmonary And Critical Care Medicine Gachon University Gil Medical Center
-
Lee Sang
Department Of Advanced Materials Science And Engineering Mokpo National University
-
Park Jeong-woong
Department Of Pulmonary And Critical Care Medicine Gachon University Gil Medical Center
-
Kim Yu
Department Of Internal Medicine Institute Of Gastroenterology Yonsei University College Of Medicine
-
Kim Yu
Department Of Pulmonary And Critical Care Medicine Gachon University Gil Medical Center
-
Kim Yu
Department Of Bioscience And Biotechnology And Department Of Chemistry Bio/molecular Informatics Cen
-
Yoon Jin
Department Of Physics Kaist
-
Kim Yu
Department Of Biochemistry College Of Science Protein Network Research Center Yonsei University
-
Yoon Jin
Department Of Ophthalmology Yonsei University College Of Medicine
-
Lee Sang
Department Of Pulmonary And Critical Care Medicine Gachon University Gil Medical Center
-
LEE Sang
Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung Hee University
関連論文
- Extracellular signal-regulated kinase (ERK) inhibition attenuates cigarette smoke extract (CSE) induced-death inducing signaling complex (DISC) formation in human lung fibroblasts (MRC-5) cells
- A Physiology Study of Escherichia coli Overexpressing Phosphoenolpyruvate Carboxykinase
- An Observation of Micro-crack in Transformation-induced Plasticity-assisted Multiphase Steel
- Investigation of the effectiveness of tandem oil fences under currents
- 自己免疫性膵炎の診断におけるIgGとIgG4の感度, 特異度 : 韓国からの報告
- Comparison of Histology and Extracellular Matrix Between Autoimmune and Alcoholic Chronic Pancreatitis
- Regression of Pancreatic Fibrosis After Steroid Therapy in Patients With Autoimmune Chronic Pancreatitis
- Studies on Effects of Impurity Doping and NMR Measurements of La 1111 and/or Nd 1111 Fe-Pnictide Superconductors
- Studies on Superconducting LaFe_Co_yAsO_F_x (x = 0.11) : For the Understanding of the Superconducting Symmetry of Fe-Pnictides
- Distinct Transport Behaviors of LaFe_Co_yAsO_F_x (x = 0.11) between the Superconducting and Nonsuperconducting Metallic y Regions Divided by y - 0.05
- Associations Between Lipid Measures and Metabolic Syndrome, Insulin Resistance and Adiponectin : Usefulness of Lipid Ratios in Korean Men and Women
- Determination of Carbamazepine by Chemiluminescence Detection Using Chemically Prepared Tris(2, 2'-bipyridine)-ruthenium (III) as Oxidant
- Prognostic Factors for the Surgery for Mesial Temporal Lobe Epilepsy : Longitudinal Analysis
- Parietal Lobe Epilepsy : The Semiology, Yield of Diagnostic Workup, and Surgical Outcome
- 2-8.Clinical Usefulness of Cervicogram as Primary Screening Test of Cervical Intraepithelial Neoplasia(Session 3 Oncology 3)
- ON COMPLETELY REGULAR AND QUASI-COMPLETELY REGULAR ORDERED SEMIGROUPS
- FDG-PET Images Quantified by Probabilistic Atlas of Brain and Surgical Prognosis of Temporal Lobe Epilepsy
- Prognostic Factors in Anterior Temporal Lobe Resections for Mesial Temporal Lobe Epilepsy : Multivariate Analysis
- Endoprosthetic Reconstruction after Resection of Primary Bone Tumors
- Microstructure and electrical properties of nano-sized Ce_Gd_xO_2 (0≤x≤0.2) particles prepared by spray pyrolysis
- Superconductivity of LaFe_Co_yAsO_F_x(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- Tilting of Liquid Crystal through Interaction with Methyl Orange Molecules Oriented by Circularly Polarized Light
- Flow Field Analysis inside a Molten Zn Pot of the Continuous Hot-dip Galvanizing Process
- Comparative evaluation of segmentation methods for bronchial tree in the lung parenchyma
- Bioflocculant Produced by Aspergillus sp. JS-42
- The Analgesic Effects of Automatically Controlled Rotating Acupuncture in Rats : Mediation by Endogenous Opioid System
- Failing to achieve a nadir prostate-specific antigen after combined androgen blockade : Predictive factors
- N-Terminal Pro-B-Type Natriuretic Peptide Predicts Significant Coronary Artery Lesion in the Unstable Angina Patients With Normal Electrocardiogram, Echocardiogram, and Cardiac Enzymes
- Methylation status of mage gene famaily and cage1 promoters in squamous cell carcinoma of uterine cervix(Oncology 4)
- Comparative measurements on the flow structure of a marine propeller wake between an open free surface and closed surface flows
- Performance of DS-CDM-SQAM in Nonlinear Land Mobile Satellite Broadcasting Environments(Components)
- Microbial Transformation of Terreusinone, an Ultraviolet-A (UV-A) Protecting Dipyrroloquinone, by Streptomyces sp.
- FMRFamide-Expressing Efferent Neurons in Eighth Abdominal Ganglion Innervate Hindgut in the Silkworm, Bombyx mori(Neurobiology)
- Effects of 20-Hydroxyecdysone and Serotonin on Neurite Growth and Survival Rate of Antennal Lobe Neurons in Pupal Stage of the Silk Moth Bombyx mori in vitro(Neurobiology)
- Rationalization of Public Forest Management Using Multiple Regression Analysis : Focused on Jeonbuk Province in Korea
- PE-184 Changes of Plasma NT-proBNP Can Predict Echocardiographic Variables after Medical Treatment in Hypertrophic Cardiomyopathy with Normal Ejection Fraction(Cardiomyopathy, basic/clinical-4 (M) PE31,Poster Session (English),The 70th Anniversary Annual
- Perineural invasion is a prognostic factor for biochemical failure after radical prostatectomy
- Interpretation of Wada Memory Test for Lateralization of Seizure Focus by Use of ^Technetium-HMPAO SPECT
- Evaluation of Functional Changes in the Medial Temporal Region During Intracarotid Amobarbital Procedure by Use of SPECT
- Juxta-clavicular beaded lines
- Raman Study of the Effects of Hydrogen Gas Annealing on PbTiO_3 Crystals
- A Case of Acute Organotin Poisoning
- p38 Mitogen-Activated Protein Kinase Regulates Vasoconstriction in Spontaneously Hypertensive Rats
- Effect of Maleated Polyethylene on the Rheological Properties of LLDPE/Clay Nanocomposites
- Remineralization of early enamel caries lesions by CPP-ACP paste : A case report
- Mulberroside F Isolated from the Leaves of Morus alba Inhibits Melanin Biosynthesis
- Outcome of the functional treatment of first-time ankle inversion injury
- Effects of Tegaserod on Ileal Peristalsis of Guinea Pig In Vitro
- MOLECULAR CLONING OF THE GENOME OF RICE RAGGED STUNT VIRUS
- Solvent Effect on Formation of Cysteamine Self-Assembled Monolayers on Au(111)
- APPLICATIONS OF CHEMICAL-MECHANICAL-POLISHING PROCESS TO SILICON FIELD EMITTER ARRAY
- Linear lichenoid graft versus host disease : An unsual configuration following Blaschko's lines
- Occipital Lobe Epilepsy : Clinical Characteristics, Surgical Outcome, and Role of Diagnostic Modalities
- Epidemiologic picture of Kawasaki disease in Korea, 2000-2002
- Cancer screening using ^F-FDG PET/CT in Korean asymptomatic volunteers : a preliminary report
- Superiority of HMPAO Ictal SPECT to ECD Ictal SPECT in Localizing the Epileptogenic Zone
- Diagnostic Performance of [^F]FDG-PET and Ictal [^Tc]-HMPAO SPECT in Occipital Lobe Epilepsy
- Disparity of Perfusion and Glucose Metabolism of Epileptogenic Zones in Temporal Lobe Epilepsy Demonstrated by SPM/SPAM Analysis on ^O Water PET, [^F]FDG-PET, and [^Tc]-HMPAO SPECT
- Body mass index influences prostate-specific antigen in men younger than 60 years of age
- Different Contractile Properties between Intralobar and Extralobar Pulmonary Arteries of the Rabbit
- NONLINEAR MAPPING DESCRIBING LEARNING PROCESS HAVING MEMORY(Session I : Cross-Disciplinary Physics, The 1st Tohwa University International Meeting on Statistical Physics Theories, Experiments and Computer Simulations)
- The 5' Flanking Sequences of Two S Alleles in Lycopersicon peruvianum Are Highly Heterologous but Contain Short Blocks of Homologous Sequences : GENE STRUCTURE AND EXPRESSION : CELL-CELL INTERACTION
- Recent transplantation situation in Korea
- Glomerular crescents are responsible for chronic graft dysfunction in post-transplant IgA nephropathy
- Effects of Carvedilol Alone and in the Presence of Cyclosporine A on the DNA Synthesis of Cultured Vascular Smooth Muscle Cells
- Long-Term Results of Kidney Transplantation Between HLA-Identical Siblings
- Systemic chemotherapy in patients with indwelling ureteral stenting
- The Clinical Usefulness of Ictal Surface EEG in Neocortical Epilepsy
- Chip equalizer using tap selection algorithm for Satellite Digital Multimedia Broadcasting (DMB) (衛星通信)
- PE-082 CABG versys PCI for Left Main Disease(Coronary revascularization, PCI-8 (IHD) PE14,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- FRS-025 Does Drug Eluting Stent Really Improved Clinical Outcomes of Percutaneous Coronary Intervention in Daily Practice?(New PCI-1 (IHD) FRS5,Featured Research Session,The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- Degree of Left Ventricular Hypertrophy Determines Plasma NT pro-BNP Level in Hypertrophic Cardiomyopathy(Cardiomyopathy, Clinical 2 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Lack of Additional Benefit of Intracoronary Transplantation of Autologous Peripheral Blood Stem Cell in Patients With Acute Myocardial Infarction
- OE-289 Virus Receptor Trap, Express Both CAR and DAF, Attenuates Inflammation and Mortality in Experimental Coxsackieviral Myocarditis(Myocarditis, basic/clinical-1 (M) OE49,Oral Presentation (English),The 70th Anniversary Annual Scientific Meeting of the
- Neurotrophin Receptor Immunoreactivity in Severe Cerebral Cortical Dysplasia
- Preload Dependency of the Time Interval Between the Onset of Mitral Inflow and the Early Diastolic Annular Motion : A Hemodialysis-Related Preload Reduction Study
- Identification of a Ribosomal Frameshift in Leishmania RNA Virus 1-4
- Annealing Temperature Dependence of the Amorphous Structure of Amorphous Si-Ge-Au Thin Films
- Anomalous Large Thermoelectric PoWer on Heavily B-Doped SiGe Thin Films with Thermal Annealing : Structure and Mechanical and Thermal Properties of Condensed Matter
- Surgical Outcome of Epilepsy Caused by Cortical Dysplasia
- The success predictor of multiple doses for ectopic tubal pregnancy treated with single-dose regimen MTX(Reproduction 4)
- Feedback control of a circular cylinder wake with rotational oscillation
- Gallbladder torsion-induced emphysematous cholecystitis in a 16-year-old boy
- Deep Middle Masseteric Artery (dMMA) Attributed to Hemorrhage in Resection of Masseter Muscle and Mandibular Angle
- Contrasting activity of Hedgehog and Wnt pathways according to gastric cancer cell differentiation : Relevance of crosstalk mechanisms
- FLOW CYTOMETRIC DETERMINATION OF THE DNA CONTENT IN TRANSITIONAL CELL CARCINOMA OF THE BLADDER : PREDICTABILITY OF PLOIDY WITH REGARD TO PELVIC LYMPH NODE METASTASIS
- Effect of Timing of Meal Intake after Squat Exercise Training on Bone Formation in the Rat Hindlimb
- Effects of amide types DCCAs on the properties of Y_2O_3:Eu phosphor powders with spherical shape and fine size
- Ethnic Differences of two Non-synonymous Single Nucleotide Polymorphisms in CDA Gene
- Long-term continuous adaptation of Escherichia coli to high succinate stress and transcriptome analysis of the tolerant strain(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- An Observation of Micro-crack in Transformation-induced Plasticity-assisted Multiphase Steel
- Beam Combination using Stimulated Brillouin Scattering for the Ultimate High Power-Energy Laser System Operating at High Repetition Rate over 10Hz for Laser Fusion Driver
- The Use of Alkaline EDTA Solution Improves Heat-induced Epitope Retrieval for Immunohistochemical Localization of MIB-1 Antigen
- Effect of erythromycin on gastric emptying in healthy individuals assessed by radio-opaque markers and plasma acetaminophen levels
- Crystal structure of Poly(hexamethylene 2,6-naphthalate)
- Fungal Melanonychia
- Successful elimination of Ascaris lumbricoides from the gallbladder by conservative medical therapy
- Tracheal Glomangiosarcoma with Multiple Skin Metastasis
- Evidence That a β-1,4-Endoglucanase Secreted by Acetobacter xylinum Plays an Essential Role for the Formation of Cellulose Fiber
- NMR Spin Relaxation Experiments for Characterizing Motions in Peptides