鉄塩誘導てんかんモデルラットの無麻酔自由行動条件下における海馬抗酸化能力の減弱 : 無麻酔自由行動条件下でのX-band ESRを用いた検討

概要

論文の詳細を見る
To analyze the hippocampal anti-oxydant ability in the Fe-induced epilepsy model, microdialysis was used to monitor the sequential changes of an exogenous nitroxide radical by X-band ESR in the freely moving rats. Nitroxide radicals used in this study are 200mM 3-methoxy carbonyl-2,2,5,5-tetramethylpyrrolidine-l-oxyl (PCAM) i.p. administration and 300mM 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-l-oxyl(carbamoyl-PROXYL) perfusated into the hippocampus for 70 min at 3ul/min. Each half-life of carbamoyl-PROXYL and PCAM in the epilepsy model was statistically longer than that of control, respectively. Prolonged half-life of carbamoyl-PROXYL reveals the decreased antioxydant ability in the extracellular space (ascorbic acid), while prolonged half-life of PCAM indicates the decreased anti-oxydant ability in the neuronal membrane (ascorbic acid and α-tocopherol). The result of the decreased hippocampal anti-oxydant ability is possibly due to lipid peroxidation induced by the repetitive seizures, and the decreased aniti-oxydant ability will results in the more severe damage in the pyramidal cell of the hippocampus.
日本医学館,Magnetic resonance in medicineの論文