βアミロイドタンパクによる培養細胞系でのラジカル発生

概要

論文の詳細を見る
There is few direct electron paramagnetic resonance (EPR) evidence of supporting the hypothesis of lipid peroxidation induced by beta amyloid. Hence in this study EPR measurement was performed in the cultured PC12 cells using two kinds of spin trapping agents (DMPO, POBN) to test the hypothesis. Spin adduct of DMPO in the cells stimulated by beta amyloid (25-35 400uM) revealed characteristically four line spectra, for which the hfc were aN=14.9G and aH=14.9G; its hfc were corresponded to DMPO-OH. The EPR signal from the PC12 cells cultured with beta amyloid were characteristically six-line spectra, for which the hfc were aN=15. 7G and aH=2.5G; its hfc were corresponded to that of lipid radical. This study presents the direct EPR evidence of hydroxyl radical formation in PC12 cell cultured with beta amyloid 25-35 peptide. Generation of hydroxyl radical results in abstraction hydrogen from polyunsaturated lipid acid, and plays a causative role in lipid peroxidation. This result might be important evidence of initiation of lipid peroxidation and the growing lipid peroxidation in the cascade of neurotoxicity induced by beta amyloid 25-35 peptide.
日本医学館,Magnetic resonance in medicineの論文