微生物に取り込まれた銅(II)イオンのESR による解析

概要

論文の詳細を見る
The copper accumulation by various microorganisms were examined. Of microorganisms tested, Arthrobacter nicotianae IAM 12342 (603 ?mol/g cells), Micrococcus luteus IAM 1056 (529 ?mol/g cells), Streptomyces flavoviridis HUT 6147 (499 ?mol/g cells), Streptomyces levoris HUT6156 (551 ?mol/g cells) have high abilities to accumulate copper. Copper accumulation bybacterial cells was so rapid, and was affected by the pH of the solution. The ESR spectra of Cu(II) ion taken up in microbial cells were axial type, having a major absorption to higher field at g⊥ and lesser absorption to lower field at g// with four lines. The ESR parameters, g// and |A//|,of these spectra were in the ranges of 2.238～2.303 and 15.6～18.5 mcm－1, respectively. The ESR spectra of Cu(II) in Arthrobacter nicotianae and Rhizopus oryzae cells were the superposition of two types of signals (A: g// = 2.245～2.252, |A//| = 17.6～18.2 mcm－1 and B: g// = 2.285～2.289, |A//| = 15.6～15.9 mcm－1). A new signal, g// = 2.388, |A//| = 13.0 mcm－1, was observed in Arthrobacter nictianae cells at pH 2～3. The relationship between g// and |A//| ofCu(II) in microbial cells are almost linear, which is a similar manner as that of Cu(II)－ glycyl-glycylglycylglycine in a series of pH values. These results suggested that Cu(II) in microbial cells were in the similar axial type coordination circum-stances with nitrogen and oxygen donors as those of type II Cu-proteins, and that donor atoms of Cu(II) in microbial cells were altered from 4N (higher pH) to 4O (lower pH) according to the pH variation of their coordination abilities.
日本医学館,Magnetic resonance in medicineの論文

微生物に取り込まれた銅(II)イオンのESR による解析

スポンサーリンク

概要

著者

関連論文

スポンサーリンク